Ser577
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Home > Phosphorylation Site Page: > Ser577  -  SIK (mouse)

Site Information
FQEGRRAsDTSLTQG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 465992

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 12 ) , mass spectrometry ( 2 , 3 , 5 , 7 , 8 , 10 , 11 ) , mutation of modification site ( 12 , 13 ) , phospho-antibody ( 9 , 12 ) , western blotting ( 9 , 12 )
Disease tissue studied:
adrenal cancer ( 13 ) , adrenocortical carcinoma ( 13 ) , neuroblastoma ( 7 ) , melanoma skin cancer ( 10 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 , 3 , 5 ) , COS (fibroblast) ( 9 , 12 ) , heart ( 8 ) , Hepa 1-6 (epithelial) ( 11 ) , kidney ( 8 ) , liver ( 8 ) , lung ( 8 ) , N1E-115 (neuron) ( 7 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 10 ) , spleen ( 8 ) , Y-1 (adrenal cortical) ( 12 , 13 )

Upstream Regulation
Putative in vivo kinases:
PKCA (mouse) ( 12 )
Kinases, in vitro:
PKACA (cow) ( 12 )
Treatments:
ACTH ( 12 , 13 ) , LPA ( 7 )

Downstream Regulation
Effects of modification on SIK:
intracellular localization ( 12 , 13 )
Effects of modification on biological processes:
transcription, altered ( 13 ) , transcription, induced ( 12 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

3

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Stewart R, et al. (2013) Regulation of SIK1 abundance and stability is critical for myogenesis. Proc Natl Acad Sci U S A 110, 117-22
23256157   Curated Info

7

Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201
21454597   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Uebi T, et al. (2010) Phosphorylation of the CREB-specific coactivator TORC2 at Ser307 regulates its intracellular localization in COS-7 cells and in the mouse liver. Am J Physiol Endocrinol Metab 299, E413-25
20551288   Curated Info

10

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

11

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

12

Takemori H, et al. (2002) ACTH-induced nucleocytoplasmic translocation of salt-inducible kinase. Implication in the protein kinase A-activated gene transcription in mouse adrenocortical tumor cells. J Biol Chem 277, 42334-43
12200423   Curated Info

13

Katoh Y, Takemori H, Doi J, Okamoto M (2002) Identification of the nuclear localization domain of salt-inducible kinase. Endocr Res 28, 315-8
12530631   Curated Info