Tyr328
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Home > Phosphorylation Site Page: > Tyr328  -  EFNB1 (mouse)

Site Information
GDyGHPVyIVQEMPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448343

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 ) , mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 ) , phospho-antibody ( 3 , 8 ) , western blotting ( 3 )
Disease tissue studied:
testicular cancer ( 2 )
Relevant cell line - cell type - tissue:
'neuron, cortical'-brain ( 8 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 8 ) , 3T3 (fibroblast) ( 3 ) , C2C12-D (muscle cell) ( 5 , 6 ) , MEF (fibroblast) ( 4 , 7 ) , P19 (testicular) ( 2 ) , SYF (fibroblast) [Src iso1 (mouse), transfection] ( 1 )

Upstream Regulation
Putative in vivo kinases:
Src iso1 (mouse) ( 8 )
Treatments:
EphB1 ( 8 ) , EphB2 ( 3 , 8 ) , EphB4 ( 8 )

References 

1

Ferrando IM, et al. (2012) Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics. Mol Cell Proteomics 11, 355-69
22499769   Curated Info

2

Zhou J (2010) CST Curation Set: 9109; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

3

Makarov R, et al. (2010) The impact of CFNS-causing EFNB1 mutations on ephrin-B1 function. BMC Med Genet 11, 98
20565770   Curated Info

4

Luo W, et al. (2008) Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res 7, 3447-60
18563927   Curated Info

5

Guo A (2007) CST Curation Set: 2782; Year: 2007; Biosample/Treatment: cell line, C2C12-D/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Guo A (2007) CST Curation Set: 2783; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Li Y (2006) CST Curation Set: 1908; Year: 2006; Biosample/Treatment: cell line, MEF/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Palmer A, et al. (2002) EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase. Mol Cell 9, 725-37
11983165   Curated Info