Tyr323

Site Information
yEkVsGDyGHPVyIV SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448342

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 6 ) , mass spectrometry ( 1 , 3 , 4 , 5 ) , phospho-antibody ( 2 , 6 ) , western blotting ( 2 )
Disease tissue studied:	testicular cancer ( 1 )
Relevant cell line - cell type - tissue:	'neuron, cortical'-brain ( 6 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 6 ) , 3T3 (fibroblast) ( 2 ) , C2C12-D (muscle cell) ( 4 , 5 ) , MEF (fibroblast) ( 3 ) , P19 (testicular) ( 1 )

Upstream Regulation
Putative in vivo kinases:	Src iso1 (mouse) ( 6 )
Treatments:	EphB1 ( 6 ) , EphB2 ( 2 , 6 ) , EphB4 ( 6 )

References
1	Zhou J (2010) CST Curation Set: 9109; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416 Curated Info
2	Makarov R, et al. (2010) The impact of CFNS-causing EFNB1 mutations on ephrin-B1 function. BMC Med Genet 11, 98 20565770 Curated Info
3	Luo W, et al. (2008) Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res 7, 3447-60 18563927 Curated Info
4	Guo A (2007) CST Curation Set: 2782; Year: 2007; Biosample/Treatment: cell line, C2C12-D/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
5	Guo A (2007) CST Curation Set: 2783; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
6	Palmer A, et al. (2002) EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase. Mol Cell 9, 725-37 11983165 Curated Info