Thr175

Site Information
RRLsFIGtPyWMAPE SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4198300

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 5 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 , 5 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 1 ) , Jurkat (T lymphocyte) ( 2 , 3 , 4 , 5 )

Downstream Regulation
Effects of modification on HPK1:	enzymatic activity, induced ( 1 , 5 ) , protein degradation ( 1 )
Effects of modification on biological processes:	transcription, altered ( 5 )

References
1	Wang H, et al. (2014) The CUL7/F-box and WD Repeat Domain Containing 8 (CUL7/Fbxw8) Ubiquitin Ligase Promotes Degradation of Hematopoietic Progenitor Kinase 1. J Biol Chem 289, 4009-17 24362026 Curated Info
2	Mulhern D (2012) CST Curation Set: 13109; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY] Curated Info
3	Guo A (2011) CST Curation Set: 11885; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Curated Info
4	Guo A (2011) CST Curation Set: 11891; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY] Curated Info
5	Arnold R, et al. (2005) Activation of hematopoietic progenitor kinase 1 involves relocation, autophosphorylation, and transphosphorylation by protein kinase D1. Mol Cell Biol 25, 2364-83 15743830 Curated Info