Ser423
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Home > Phosphorylation Site Page: > Ser423  -  SMAD3 (mouse)

Site Information
SPSIRCSsVs_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448335

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 4 ) , immunoassay ( 1 , 2 ) , phospho-antibody ( 1 , 3 , 5 ) , western blotting ( 1 , 2 , 3 , 5 )
Relevant cell line - cell type - tissue:
bone marrow ( 5 ) , bone marrow [SMAD3 (mouse), homozygous knockout] ( 5 ) , C2C12 (myoblast) ( 2 ) , heart ( 1 ) , hematopoietic stem cells ( 4 ) , lung ( 1 , 3 )

Upstream Regulation
Regulatory protein:
caveolin-2 (mouse) ( 3 ) , POSTN (mouse) ( 1 ) , TDGF1 (mouse) ( 4 ) , TGFB1 (mouse) ( 1 )
Treatments:
bleomycin ( 3 ) , hypoxia ( 1 ) , nusinersen ( 2 ) , SB431542 ( 2 ) , TGF-beta ( 5 )

Downstream Regulation
Effects of modification on SMAD3:
intracellular localization ( 2 ) , molecular association, regulation ( 2 )
Effects of modification on biological processes:
transcription, induced ( 2 )
Induce interaction with:
DNA ( 2 )

References 

1

Seki M, et al. (2019) Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension. PLoS One 14, e0220795
31437169   Curated Info

2

Aloysius A, DasGupta R, Dhawan J (2018) The transcription factor Lef1 switches partners from β-catenin to Smad3 during muscle stem cell quiescence. Sci Signal 11
30042129   Curated Info

3

de Almeida CJ, Jasmin JF, Del Galdo F, Lisanti MP (2013) Genetic ablation of caveolin-2 sensitizes mice to bleomycin-induced injury. Cell Cycle 12, 2248-54
24067367   Curated Info

4

Miharada K, et al. (2011) Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78. Cell Stem Cell 9, 330-44
21982233   Curated Info

5

Fujita T, et al. (2008) Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells. Mol Biol Cell 19, 5446-55
18843049   Curated Info