Tyr567
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Home > Phosphorylation Site Page: > Tyr567  -  NPM-ALK (human)

Site Information
TSLWNPTyGSWFTEK   GenPept
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 27275100
Associated spectra:  6 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 4 , 5 , 7 )
Disease tissue studied:
neuroblastoma ( 2 )
Relevant cell line - cell type - tissue:

Downstream Regulation
Effects of modification on NPM-ALK:
molecular association, regulation ( 4 , 7 )
Effects of modification on biological processes:
transcription, induced ( 5 )
Induce interaction with:
SHC1 (mouse) ( 7 ) , SNTB1 (human) ( 4 )

References 

1

Wang P, et al. (2011) Serine phosphorylation of NPM-ALK, which is dependent on the auto-activation of the kinase activation loop, contributes to its oncogenic potential. Carcinogenesis 32, 146-53
21045017   Curated Info

2

Guo A (2010) CST Curation Set: 10556; Year: 2010; Biosample/Treatment: cell line, SH-SY5Y/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Wang P, et al. (2010) Functional characterization of the kinase activation loop in nucleophosmin (NPM)-anaplastic lymphoma kinase (ALK) using tandem affinity purification and liquid chromatography-mass spectrometry. J Biol Chem 285, 95-103
19887368   Curated Info

4

Chikamori M, Fujimoto J, Tokai-Nishizumi N, Yamamoto T (2007) Identification of multiple SNT-binding sites on NPM-ALK oncoprotein and their involvement in cell transformation. Oncogene 26, 2950-4
17086210   Curated Info

5

Turner SD, et al. (2007) The NPM-ALK tyrosine kinase mimics TCR signalling pathways, inducing NFAT and AP-1 by RAS-dependent mechanisms. Cell Signal 19, 740-7
17110082   Curated Info

6

Bischof D, Pulford K, Mason DY, Morris SW (1997) Role of the nucleophosmin (NPM) portion of the non-Hodgkin's lymphoma-associated NPM-anaplastic lymphoma kinase fusion protein in oncogenesis. Mol Cell Biol 17, 2312-25
9121481   Curated Info

7

Fujimoto J, et al. (1996) Characterization of the transforming activity of p80, a hyperphosphorylated protein in a Ki-1 lymphoma cell line with chromosomal translocation t(2;5). Proc Natl Acad Sci U S A 93, 4181-6
8633037   Curated Info