Thr1074
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr1074  -  GLI1 (human)

Site Information
QRGSsGHtPPPsGPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 41595601

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 3 , 4 ) , mass spectrometry ( 3 , 4 ) , mutation of modification site ( 1 , 3 , 4 ) , phospho-antibody ( 1 , 2 , 3 , 4 ) , western blotting ( 1 , 2 , 3 , 4 )
Disease tissue studied:
brain cancer ( 1 ) , medulloblastoma ( 1 ) , lymphoma ( 3 ) , B cell lymphoma ( 3 ) , non-Hodgkin's lymphoma ( 3 ) , follicular lymphoma ( 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 ) , 293T (epithelial) ( 1 ) , 3T3 (fibroblast) ( 1 , 2 , 4 ) , Daoy ( 1 ) , HEK193T (epithelial) ( 2 ) , MEF (fibroblast) ( 2 ) , OCI-ly10 (B lymphocyte) ( 3 ) , OCI-ly3 (B lymphocyte) ( 3 ) , SU-DHL-4 (B lymphocyte) ( 3 )

Upstream Regulation
Putative in vivo kinases:
AMPKA2 (human) ( 4 ) , MEKK3 (human) ( 1 )
Kinases, in vitro:
AMPKA2 (human) ( 4 )
Treatments:
A79662 ( 4 ) , acadesine ( 4 )

Downstream Regulation
Effects of modification on GLI1:
activity, inhibited ( 4 ) , intracellular localization ( 1 , 2 ) , molecular association, regulation ( 1 , 2 ) , protein degradation ( 2 , 4 ) , ubiquitination ( 2 )
Effects of modification on biological processes:
carcinogenesis, inhibited ( 4 ) , cell growth, inhibited ( 4 ) , transcription, inhibited ( 1 )
Induce interaction with:
BTRC (human) ( 2 ) , SUFU (human) ( 1 )
Inhibit interaction with:
DNA ( 1 )

References 

1

Lu J, et al. (2018) MEKK2 and MEKK3 suppress Hedgehog pathway-dependent medulloblastoma by inhibiting GLI1 function. Oncogene
29662197   Curated Info

2

Zhang R, et al. (2017) Dual degradation signals destruct GLI1: AMPK inhibits GLI1 through β-TrCP-mediated proteasome degradation. Oncotarget 8, 49869-49881
28562331   Curated Info

3

Agarwal NK, et al. (2016) Active IKKβ promotes the stability of GLI1 oncogene in diffuse large B-cell lymphoma. Blood 127, 605-15
26603838   Curated Info

4

Li YH, et al. (2015) AMP-Activated Protein Kinase Directly Phosphorylates and Destabilizes Hedgehog Pathway Transcription Factor GLI1 in Medulloblastoma. Cell Rep 12, 599-609
26190112   Curated Info