Lys386
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Home > Ubiquitylation Site Page: > Lys386  -  p53 (human)

Site Information
RHkkLMFktEGPDsD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 464662

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 5 ) , mass spectrometry ( 3 ) , mutation of modification site ( 5 , 6 , 7 ) , western blotting ( 5 , 6 , 7 )
Disease tissue studied:
bone cancer ( 5 , 6 , 7 ) , lung cancer ( 6 ) , non-small cell lung cancer ( 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Treatments:
MG132 ( 6 )

Downstream Regulation
Effects of modification on p53:
intracellular localization ( 5 ) , protein degradation ( 5 , 6 )
Effects of modification on biological processes:
transcription, induced ( 7 ) , transcription, inhibited ( 6 )

References 

1

Thalappilly S, et al. (2011) The p53 tumor suppressor is stabilized by inhibitor of growth 1 (ING1) by blocking polyubiquitination. PLoS One 6, e21065
21731648   Curated Info

2

Marchenko ND, et al. (2010) Stress-mediated nuclear stabilization of p53 is regulated by ubiquitination and importin-alpha3 binding. Cell Death Differ 17, 255-67
19927155   Curated Info

3

Guo A (2009) CST Curation Set: 8633; Year: 2009; Biosample/Treatment: cell line, AMO-1/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

4

Okamoto K, Taya Y, Nakagama H (2009) Mdmx enhances p53 ubiquitination by altering the substrate preference of the Mdm2 ubiquitin ligase. FEBS Lett 583, 2710-4
19619542   Curated Info

5

Nie L, Sasaki M, Maki CG (2007) Regulation of p53 nuclear export through sequential changes in conformation and ubiquitination. J Biol Chem 282, 14616-25
17371868   Curated Info

6

Rodriguez MS, et al. (2000) Multiple C-terminal lysine residues target p53 for ubiquitin-proteasome-mediated degradation. Mol Cell Biol 20, 8458-67
11046142   Curated Info

7

Rodriguez MS, et al. (1999) SUMO-1 modification activates the transcriptional response of p53. EMBO J 18, 6455-61
10562557   Curated Info