Thr286
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Home > Phosphorylation Site Page: > Thr286  -  CCND1 (mouse)

Site Information
EEAGLACtPtDVRDV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447839

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 11 ) , [32P] ATP in vitro ( 10 ) , [32P] bio-synthetic labeling ( 7 , 10 ) , flow cytometry ( 4 ) , immunoassay ( 10 ) , immunoprecipitation ( 10 , 11 ) , mass spectrometry ( 2 ) , mutation of modification site ( 5 , 7 , 8 , 9 , 10 , 11 ) , phospho-antibody ( 3 , 4 , 6 , 9 ) , phosphopeptide mapping ( 11 ) , western blotting ( 3 , 4 , 10 , 11 )
Disease tissue studied:
melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 6 , 9 ) , 3T3 (fibroblast) ( 4 , 10 ) , C2C12 (myoblast) ( 5 ) , MEF (fibroblast) [IGF1R (mouse)] ( 8 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 2 ) , melanocyte-skin [IKKB (mouse), homozygous knockout] ( 3 ) , Mv1Lu (pulmonary) ( 7 ) , SF9 ( 10 , 11 )

Upstream Regulation
Regulatory protein:
CCND1 (mouse) ( 4 ) , IKKB (mouse) ( 3 )
Putative in vivo kinases:
DYRK1B (mouse) ( 7 ) , GSK3A (human) ( 10 ) , GSK3B (mouse) ( 10 )
Kinases, in vitro:
DYRK1B (mouse) ( 7 ) , GSK3B (mouse) ( 7 , 9 , 10 )
Treatments:
KU-55933 ( 4 ) , lithium ( 7 , 9 ) , MG132 ( 4 ) , myostatin ( 5 ) , neocarzinostatin ( 4 ) , siRNA ( 4 ) , UV ( 4 )

Downstream Regulation
Effects of modification on CCND1:
activity, induced ( 8 ) , intracellular localization ( 8 , 9 , 10 ) , molecular association, regulation ( 8 ) , protein degradation ( 5 , 6 , 10 , 11 ) , protein stabilization ( 8 ) , ubiquitination ( 11 )
Effects of modification on biological processes:
cell cycle regulation ( 4 , 9 )
Induce interaction with:
PIN1 (mouse) ( 8 )

References 

1

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

2

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

3

Yang J, et al. (2010) Conditional ablation of Ikkb inhibits melanoma tumor development in mice. J Clin Invest 120, 2563-74
20530876   Curated Info

4

Hitomi M, Yang K, Stacey AW, Stacey DW (2008) Phosphorylation of cyclin D1 regulated by ATM or ATR controls cell cycle progression. Mol Cell Biol 28, 5478-93
18606783   Curated Info

5

Yang W, et al. (2007) Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem 282, 3799-808
17130121   Curated Info

6

Guo Y, et al. (2005) Phosphorylation of cyclin D1 at Thr 286 during S phase leads to its proteasomal degradation and allows efficient DNA synthesis. Oncogene 24, 2599-612
15735756   Curated Info

7

Zou Y, et al. (2004) Mirk/dyrk1B kinase destabilizes cyclin D1 by phosphorylation at threonine 288. J Biol Chem 279, 27790-8
15075324   Curated Info

8

Liou YC, et al. (2002) Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes. Proc Natl Acad Sci U S A 99, 1335-40
11805292   Curated Info

9

Alt JR, Cleveland JL, Hannink M, Diehl JA (2000) Phosphorylation-dependent regulation of cyclin D1 nuclear export and cyclin D1-dependent cellular transformation. Genes Dev 14, 3102-14
11124803   Curated Info

10

Diehl JA, Cheng M, Roussel MF, Sherr CJ (1998) Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev 12, 3499-511
9832503   Curated Info

11

Diehl JA, Zindy F, Sherr CJ (1997) Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway. Genes Dev 11, 957-72
9136925   Curated Info