Lys124
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Home > Ubiquitylation Site Page: > Lys124  -  CENPA (human)

Site Information
GRVTLFPkDVQLARR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 40439801

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 3 ) , mass spectrometry ( 1 , 3 ) , mutation of modification site ( 1 , 2 , 3 , 5 ) , western blotting ( 1 , 2 , 3 , 5 )
Disease tissue studied:
colorectal cancer ( 2 ) , colorectal carcinoma ( 2 )
Relevant cell line - cell type - tissue:
DLD1 (intestinal) ( 2 ) , HEK293T (epithelial) ( 1 , 3 ) , HeLa (cervical) ( 1 , 3 , 5 ) , RPE-1 (retinal) ( 2 , 3 )

Upstream Regulation
Regulatory protein:
COPS8 (human) ( 5 )

Downstream Regulation
Effects of modification on CENPA:
intracellular localization ( 1 , 3 , 5 ) , molecular association, regulation ( 5 ) , protein degradation ( 1 ) , ubiquitination ( 3 )
Effects of modification on biological processes:
cell cycle regulation ( 1 )
Induce interaction with:
HJURP (human) ( 5 )

References 

1

Wang K, et al. (2021) Phosphorylation at Ser68 facilitates DCAF11-mediated ubiquitination and degradation of CENP-A during the cell cycle. Cell Rep 37, 109987
34758320   Curated Info

2

Salinas-Luypaert C, et al. (2021) Gene replacement strategies validate the use of functional tags on centromeric chromatin and invalidate an essential role for CENP-A. Cell Rep 37, 109924
34731637   Curated Info

3

Niikura Y, Kitagawa R, Fang L, Kitagawa K (2019) CENP-A Ubiquitylation Is Indispensable to Cell Viability. Dev Cell 50, 683-689.e6
31550462   Curated Info

4

Fachinetti D, et al. (2017) CENP-A Modifications on Ser68 and Lys124 Are Dispensable for Establishment, Maintenance, and Long-Term Function of Human Centromeres. Dev Cell 40, 104-113
28073008   Curated Info

5

Niikura Y, et al. (2015) CENP-A K124 Ubiquitylation Is Required for CENP-A Deposition at the Centromere. Dev Cell 32, 589-603
25727006   Curated Info

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