Ser400
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Home > Phosphorylation Site Page: > Ser400  -  Cot (mouse)

Site Information
EDQPRCQsLDSALFE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447712

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 2 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 2 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 , 2 ) , macrophage-bone marrow ( 1 ) , macrophage-bone marrow [Cot (mouse)] ( 2 ) , macrophage-bone marrow [NFkB-p105 (human)] ( 2 ) , macrophage-bone marrow [NFkB-p105 (mouse)] ( 2 ) , RAW 264 (macrophage) ( 2 )

Upstream Regulation
Regulatory protein:
Cot (mouse) ( 2 ) , IKKB (human) ( 1 ) , NFkB-p105 (mouse) ( 2 )
Treatments:
IL-1b ( 1 ) , LPS ( 2 ) , LY294002 ( 2 ) , wortmannin ( 2 )

Downstream Regulation
Effects of modification on Cot:
activity, induced ( 2 ) , molecular association, regulation ( 1 ) , phosphorylation ( 1 )
Effects of modification on biological processes:
signaling pathway regulation ( 1 )
Induce interaction with:
14-3-3 gamma (human) ( 1 ) , ERK2 (mouse) ( 2 )

References 

1

Ben-Addi A, et al. (2014) IκB kinase-induced interaction of TPL-2 kinase with 14-3-3 is essential for Toll-like receptor activation of ERK-1 and -2 MAP kinases. Proc Natl Acad Sci U S A 111, E2394-403
24912162   Curated Info

2

Robinson MJ, et al. (2007) Phosphorylation of TPL-2 on serine 400 is essential for lipopolysaccharide activation of extracellular signal-regulated kinase in macrophages. Mol Cell Biol 27, 7355-64
17709378   Curated Info