Lys54

Site Information
IMRVNVDkVLERDQk SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3933158

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 )
Disease tissue studied:	leukemia ( 17 ) , testicular cancer ( 4 , 5 )
Relevant cell line - cell type - tissue:	'brain, embryonic' ( 23 ) , 'stem, embryonic' ( 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , brain ( 1 , 24 ) , ES-J1 (stem) ( 3 ) , F9 (testicular) ( 4 ) , heart ( 1 ) , kidney ( 1 ) , liver ( 1 ) , muscle ( 1 , 16 ) , P19 (testicular) ( 5 ) , pooled tissues ( 1 ) , PYS-2 (epithelial) ( 2 ) , RAW 264.7 (macrophage) ( 17 ) , spleen ( 13 ) , testis ( 14 ) , thymus ( 15 )

References
1	Wagner SA, et al. (2012) Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Mol Cell Proteomics 11, 1578-85 22790023 Curated Info
2	Zhou J (2010) CST Curation Set: 9134; Year: 2010; Biosample/Treatment: cell line, PY2/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
3	Zhou J (2010) CST Curation Set: 9129; Year: 2010; Biosample/Treatment: cell line, ES J1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
4	Zhou J (2010) CST Curation Set: 9130; Year: 2010; Biosample/Treatment: cell line, F9/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
5	Zhou J (2010) CST Curation Set: 9133; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
6	Tucker M (2010) CST Curation Set: 9089; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
7	Tucker M (2010) CST Curation Set: 9088; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
8	Tucker M (2010) CST Curation Set: 9087; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
9	Tucker M (2010) CST Curation Set: 9086; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
10	Tucker M (2010) CST Curation Set: 9084; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
11	Tucker M (2010) CST Curation Set: 9083; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
12	Tucker M (2010) CST Curation Set: 9090; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
13	Guo A (2009) CST Curation Set: 8159; Year: 2009; Biosample/Treatment: tissue, spleen/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
14	Guo A (2009) CST Curation Set: 8160; Year: 2009; Biosample/Treatment: tissue, testis/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
15	Guo A (2009) CST Curation Set: 8161; Year: 2009; Biosample/Treatment: tissue, thymus/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
16	Guo A (2009) CST Curation Set: 8158; Year: 2009; Biosample/Treatment: tissue, muscle/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
17	Possemato A (2009) CST Curation Set: 6916; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/untreated; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
18	Guo A (2008) CST Curation Set: 4122; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
19	Guo A (2008) CST Curation Set: 4124; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
20	Guo A (2008) CST Curation Set: 4127; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
21	Guo A (2008) CST Curation Set: 4128; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
22	Guo A (2008) CST Curation Set: 4129; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
23	Guo A (2007) CST Curation Set: 3116; Year: 2007; Biosample/Treatment: tissue, brain, embryonic/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info
24	Guo A (2007) CST Curation Set: 3117; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info