Ser321
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Home > Phosphorylation Site Page: > Ser321  -  CDC25B (mouse)

Site Information
QRLFRsPsMPCSVIR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448409

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 , 6 ) , mass spectrometry (in vitro) ( 5 ) , mutation of modification site ( 4 , 5 , 7 ) , phospho-antibody ( 7 ) , western blotting ( 7 )
Disease tissue studied:
testicular cancer ( 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
KRas (mouse) ( 3 )
Putative in vivo kinases:
PKACA (mouse) ( 4 , 5 , 7 )
Kinases, in vitro:
PKACA (human) ( 5 )
Treatments:
cAMP_analog ( 7 )

Downstream Regulation
Effects of modification on CDC25B:
intracellular localization ( 5 ) , phosphorylation ( 7 )
Effects of modification on biological processes:
cell cycle regulation ( 4 , 5 , 7 )

References 

1

Zhao X, et al. (2015) Successive recruitment of p-CDC25B-Ser351 and p-cyclin B1-Ser123 to centrosomes contributes to the release of mouse oocytes from prophase I arrest. Dev Dyn 244, 110-21
25349079   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Gnad F, et al. (2013) Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics. Mol Cell Proteomics 12, 2070-80
23608596   Curated Info

4

Xiao JY, Liu C, Sun XH, Yu BZ (2012) [PKA-regulated phosphorylation status of S149 and S321 sites of CDC25B inhibits mitosis of fertilized mouse eggs]. Sheng Li Xue Bao 64, 33-40
22348958   Curated Info

5

Xiao J, et al. (2011) Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs. J Biol Chem 286, 10356-66
21212267   Curated Info

6

Zhou J (2010) CST Curation Set: 9671; Year: 2010; Biosample/Treatment: cell line, F9/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

7

Cui C, et al. (2008) CDC25B acts as a potential target of PRKACA in fertilized mouse eggs. Biol Reprod 79, 991-8
18633139   Curated Info