Ser222
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Home > Phosphorylation Site Page: > Ser222  -  MEK2 (mouse)

Site Information
VsGQLIDsMANsFVG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448073

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 4 , 9 ) , phospho-antibody ( 1 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ) , western blotting ( 1 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 )
Disease tissue studied:
melanoma skin cancer ( 6 ) , type 2 diabetes ( 1 )
Relevant cell line - cell type - tissue:
'brain, striatum' ( 12 ) , 32D (myeloid) ( 16 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 15 ) , 3T3 (fibroblast) ( 14 ) , B16F1 (melanocyte) ( 6 ) , BaF3 ('B lymphocyte, precursor') ( 16 ) , brain ( 4 , 9 ) , brain [DARPP-32 (mouse), homozygous knockout] ( 13 ) , colon ( 8 ) , COS (fibroblast) ( 14 ) , embryo ( 4 ) , liver ( 4 ) , MC/9 (mast) ( 16 ) , MEF (fibroblast) ( 6 , 10 ) , myocyte-heart ( 11 ) , T lymphocyte [FOXO1A (mouse), transgenic] ( 7 )

Upstream Regulation
Regulatory protein:
CDK5R1 (mouse) ( 12 ) , DARPP-32 (mouse) ( 13 ) , eNOS (mouse) ( 11 ) , FOXP1 (mouse) ( 7 ) , IL17RD (mouse) ( 15 ) , PAG (rat) ( 10 )
Treatments:
5'-methylthioadenosine ( 6 ) , anti-CD3/CD28 ( 7 ) , bacterial infection ( 8 ) , cocaine ( 12 ) , DDATHF ( 6 ) , DETA-NONOate ( 11 ) , EGF ( 14 ) , FGF2 ( 15 ) , HGF ( 6 ) , IL-3 ( 16 ) , IL-4 ( 16 ) , IL-9 ( 16 ) , ischemia/reperfusion ( 11 ) , leucine ( 6 ) , levamfetamine ( 13 ) , nocodazole ( 14 ) , U0126 ( 6 )

References 

1

Banks AS, et al. (2015) An ERK/Cdk5 axis controls the diabetogenic actions of PPARγ. Nature 517, 391-5
25409143   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

4

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

5

Packer LM, et al. (2011) Nilotinib and MEK inhibitors induce synthetic lethality through paradoxical activation of RAF in drug-resistant chronic myeloid leukemia. Cancer Cell 20, 715-27
22169110   Curated Info

6

Andreu-Pérez P, et al. (2011) Protein arginine methyltransferase 5 regulates ERK1/2 signal transduction amplitude and cell fate through CRAF. Sci Signal 4, ra58
21917714   Curated Info

7

Feng X, et al. (2011) Transcription factor Foxp1 exerts essential cell-intrinsic regulation of the quiescence of naive T cells. Nat Immunol 12, 544-50
21532575   Curated Info

8

Chandrakesan P, et al. (2010) Novel changes in NF-{kappa}B activity during progression and regression phases of hyperplasia: role of MEK, ERK, and p38. J Biol Chem 285, 33485-98
20710027   Curated Info

9

Zhou J (2009) CST Curation Set: 7443; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

10

Oneyama C, et al. (2008) The lipid raft-anchored adaptor protein Cbp controls the oncogenic potential of c-Src. Mol Cell 30, 426-36
18498747   Curated Info

11

Xuan YT, et al. (2007) Endothelial nitric oxide synthase plays an obligatory role in the late phase of ischemic preconditioning by activating the protein kinase C epsilon p44/42 mitogen-activated protein kinase pSer-signal transducers and activators of transcription1/3 pathway. Circulation 116, 535-44
17606840   Curated Info

12

Takahashi S, et al. (2005) Increased activity of cyclin-dependent kinase 5 leads to attenuation of cocaine-mediated dopamine signaling. Proc Natl Acad Sci U S A 102, 1737-42
15665076   Curated Info

13

Valjent E, et al. (2005) Regulation of a protein phosphatase cascade allows convergent dopamine and glutamate signals to activate ERK in the striatum. Proc Natl Acad Sci U S A 102, 491-6
15608059   Curated Info

14

Hayne C, Xiang X, Luo Z (2004) MEK inhibition and phosphorylation of serine 4 on B23 are two coincident events in mitosis. Biochem Biophys Res Commun 321, 675-80
15358159   Curated Info

15

Kovalenko D, et al. (2003) Sef inhibits fibroblast growth factor signaling by inhibiting FGFR1 tyrosine phosphorylation and subsequent ERK activation. J Biol Chem 278, 14087-91
12604616   Curated Info

16

Demoulin JB, et al. (2003) MAP kinase activation by interleukin-9 in lymphoid and mast cell lines. Oncogene 22, 1763-70
12660812   Curated Info