Ser295
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Home > Phosphorylation Site Page: > Ser295  -  MEK2 (human)

Site Information
EGEPHsIsPRPRPPG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3822601
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 10 , 11 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 )
Disease tissue studied:
breast cancer ( 4 , 10 ) , breast ductal carcinoma ( 4 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , cervical cancer ( 17 ) , cervical adenocarcinoma ( 17 ) , lung cancer ( 5 , 6 , 7 , 10 ) , non-small cell lung cancer ( 6 , 7 , 10 ) , non-small cell lung adenocarcinoma ( 5 , 6 , 7 ) , non-small cell squamous cell lung carcinoma ( 5 ) , ovarian cancer ( 4 ) , melanoma skin cancer ( 2 )
Relevant cell line - cell type - tissue:
A498 (renal) ( 16 ) , breast ( 1 , 4 ) , BT-20 (breast cell) ( 10 ) , BT-549 (breast cell) ( 10 ) , Cal-12T (pulmonary) ( 7 ) , Calu 6 (pulmonary) ( 10 ) , DMS153 (pulmonary) ( 6 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 11 ) , Flp-In T-Rex-293 (epithelial) ( 11 ) , H2009 (pulmonary) ( 10 ) , H2077 (pulmonary) ( 10 ) , H2887 (pulmonary) ( 10 ) , H322M (pulmonary) ( 10 ) , HCC15 (pulmonary) ( 5 ) , HCC1937 (breast cell) ( 10 ) , HCC2279 (pulmonary) ( 10 ) , HCC44 (pulmonary) ( 7 ) , HCT116 (intestinal) ( 18 ) , HeLa (cervical) ( 3 , 13 , 19 , 21 , 22 ) , HeLa S3 (cervical) [PLK1 (human), knockdown, Tet-inducible PLK1 siRNA] ( 14 ) , HeLa S3 (cervical) ( 14 , 17 , 20 ) , HeLa_Meta (cervical) ( 15 ) , HeLa_Pro (cervical) ( 15 ) , HeLa_Telo (cervical) ( 15 ) , Jurkat (T lymphocyte) ( 8 ) , LOU-NH91 (squamous) ( 5 ) , lung ( 5 , 6 , 7 ) , MCF-7 (breast cell) ( 10 ) , MDA-MB-231 (breast cell) ( 10 ) , MDA-MB-435S (breast cell) ( 18 ) , MDA-MB-468 (breast cell) ( 10 ) , NCI-H1395 (pulmonary) ( 10 ) , NCI-H1437 (pulmonary) ( 6 , 7 ) , NCI-H1568 (pulmonary) ( 10 ) , NCI-H157 (pulmonary) ( 10 ) , NCI-H1648 (pulmonary) ( 10 ) , NCI-H1666 (pulmonary) ( 7 , 10 ) , NCI-H1703 (squamous) ( 5 ) , NCI-H2030 (pulmonary) ( 10 ) , NCI-H2073 (pulmonary) ( 5 , 6 ) , NCI-H209 (pulmonary) ( 6 ) , NCI-H2172 (pulmonary) ( 10 ) , NCI-H2342 (pulmonary) ( 5 ) , NCI-H2405 (pulmonary) ( 7 ) , NCI-H520 (squamous) ( 10 ) , NCI-H647 (pulmonary) ( 10 ) , NCI-H838 (pulmonary) ( 6 ) , ovary ( 4 ) , PC9 (pulmonary) ( 10 ) , WM239A (melanocyte) ( 2 )

Upstream Regulation
Treatments:
nocodazole ( 17 , 20 ) , selumetinib ( 2 ) , thymidine ( 20 ) , vemurafenib ( 2 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Rikova K, Hall B (2013) CST Curation Set: 20739, 21166, 30167, 30168, 30169; Year: 2013; Biosample/Treatment: cell line, H2342, H2073, Lou-NH91, HCC15, H1703; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

6

Rikova K, Hall B (2013) CST Curation Set: 20742, 21169, 30176, 30177, 30178; Year: 2013; Biosample/Treatment: cell line, H838, DMS153, H2073, H209, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

7

Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

8

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

9

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

10

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

11

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

12

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

13

Grosstessner-Hain K, et al. (2011) Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics 10, M111.008540
21857030   Curated Info

14

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

15

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

16

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

17

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

18

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

19

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
19276368   Curated Info

20

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

21

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

22

Cantin GT, et al. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res 7, 1346-51
18220336   Curated Info