Thr172
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Home > Phosphorylation Site Page: > Thr172  -  AMPKA2 (rat)

Site Information
SDGEFLRtsCGSPNY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448026

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 3 , 8 ) , mutation of modification site ( 8 , 11 ) , phospho-antibody ( 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ) , western blotting ( 2 , 4 , 5 , 6 , 7 , 9 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 9 , 10 ) , 3T3-L1 (fibroblast) ( 4 ) , adipose tissue ( 5 ) , C2C12 (myoblast) ( 4 ) , Chang liver (cervical) [AMPKB1 (rat)] ( 11 ) , Chang liver (cervical) [AMPKG1 (rat)] ( 11 ) , Chang liver (cervical) ( 8 ) , heart ( 7 ) , hepatocyte ( 2 ) , L6 (myoblast) ( 4 ) , liver ( 3 ) , myocyte-heart ( 6 )

Upstream Regulation
Regulatory protein:
Akt1 (rat) ( 6 ) , LKB1 (human) ( 6 )
Kinases, in vitro:
LKB1 (mouse) ( 8 )
Treatments:
A-769662 ( 1 ) , acadesine ( 1 , 4 ) , clozapine ( 2 ) , electrical_stimulation ( 10 ) , exercise ( 5 , 9 , 10 ) , exercise training ( 9 ) , hypoxia ( 6 ) , insulin ( 7 ) , ischemia ( 7 ) , olanzapine ( 2 ) , racepinefrine ( 5 )

Downstream Regulation
Effects of modification on AMPKA2:
enzymatic activity, induced ( 5 , 10 , 11 )

References 

1

Stone JD, et al. (2013) AMP-activated protein kinase inhibits vascular smooth muscle cell proliferation and migration and vascular remodeling following injury. Am J Physiol Heart Circ Physiol 304, H369-81
23203966   Curated Info

2

Oh KJ, et al. (2011) Atypical antipsychotic drugs perturb AMPK-dependent regulation of hepatic lipid metabolism. Am J Physiol Endocrinol Metab 300, E624-32
21224484   Curated Info

3

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

4

Suzuki A, et al. (2007) Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase. Mol Cell Biol 27, 4317-27
17420279   Curated Info

5

Koh HJ, et al. (2007) Adrenaline is a critical mediator of acute exercise-induced AMP-activated protein kinase activation in adipocytes. Biochem J 403, 473-81
17253964   Curated Info

6

Soltys CL, Kovacic S, Dyck JR (2006) Activation of cardiac AMP-activated protein kinase by LKB1 expression or chemical hypoxia is blunted by increased Akt activity. Am J Physiol Heart Circ Physiol 290, H2472-9
16428351   Curated Info

7

Horman S, et al. (2006) Insulin antagonizes ischemia-induced Thr172 phosphorylation of AMP-activated protein kinase alpha-subunits in heart via hierarchical phosphorylation of Ser485/491. J Biol Chem 281, 5335-40
16340011   Curated Info

8

Woods A, et al. (2003) Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis. J Biol Chem 278, 28434-42
12764152   Curated Info

9

Durante PE, et al. (2002) Effects of endurance training on activity and expression of AMP-activated protein kinase isoforms in rat muscles. Am J Physiol Endocrinol Metab 283, E178-86
12067859   Curated Info

10

Park SH, et al. (2002) Phosphorylation-activity relationships of AMPK and acetyl-CoA carboxylase in muscle. J Appl Physiol 92, 2475-82
12015362   Curated Info

11

Stein SC, et al. (2000) The regulation of AMP-activated protein kinase by phosphorylation. Biochem J 345 Pt 3, 437-43
10642499   Curated Info