Thr371
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr371  -  TRF1 (human)

Site Information
VSKsQPVtPEKHRAR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3727647
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 8 ) , immunoassay ( 3 , 8 ) , mass spectrometry ( 2 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ) , mutation of modification site ( 1 , 3 , 8 , 12 ) , phospho-antibody ( 1 , 3 , 8 ) , western blotting ( 1 , 8 , 12 )
Disease tissue studied:
bone cancer ( 1 ) , breast cancer ( 2 , 3 ) , breast ductal carcinoma ( 3 ) , breast cancer, triple negative ( 2 , 3 ) , fibrosarcoma of soft tissue ( 3 , 8 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
53BP1 (human) ( 3 ) , BRCA1 (human) ( 3 ) , RIF1 (human) ( 3 )
Putative in vivo kinases:
CDK1 (human) ( 3 , 8 )
Kinases, in vitro:
CDK1 (human) ( 8 , 12 )
Treatments:
CGP74514A ( 3 , 8 ) , cycloheximide ( 8 ) , DMSO ( 8 ) , KCl ( 8 ) , mirin ( 3 ) , nocodazole ( 8 ) , okadaic_acid ( 8 ) , seliciclib ( 3 , 8 )

Downstream Regulation
Effects of modification on TRF1:
intracellular localization ( 1 ) , molecular association, regulation ( 3 , 8 ) , phosphorylation ( 3 , 12 ) , protein stabilization ( 8 )
Effects of modification on biological processes:
apoptosis, induced ( 8 ) , cell cycle regulation ( 3 , 8 ) , cell growth, induced ( 8 ) , chromatin organization, altered ( 8 ) , DNA repair, induced ( 3 )
Induce interaction with:
53BP1 (human) ( 3 ) , DNA ( 3 ) , H2AX (human) ( 3 )
Inhibit interaction with:
DNA ( 8 ) , TINF2 (human) ( 8 )

References 

1

Wilson FR, Ho A, Walker JR, Zhu XD (2016) Cdk-dependent phosphorylation regulates TRF1 recruitment to PML bodies and promotes C-circle production in ALT cells. J Cell Sci 129, 2559-72
27185864   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

McKerlie M, et al. (2013) Phosphorylated (pT371)TRF1 is recruited to sites of DNA damage to facilitate homologous recombination and checkpoint activation. Nucleic Acids Res 41, 10268-82
23997120   Curated Info

4

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

5

Guo A (2011) CST Curation Set: 12435; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

6

Guo A (2011) CST Curation Set: 12436; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

7

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

8

McKerlie M, Zhu XD (2011) Cyclin B-dependent kinase 1 regulates human TRF1 to modulate the resolution of sister telomeres. Nat Commun 2, 371
21712819   Curated Info

9

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

10

Possemato A (2009) CST Curation Set: 6922; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

11

Possemato A (2008) CST Curation Set: 5276; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

12

Wu ZQ, Yang X, Weber G, Liu X (2008) Plk1 phosphorylation of TRF1 is essential for its binding to telomeres. J Biol Chem 283, 25503-13
18625707   Curated Info