Ser133
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Home > Phosphorylation Site Page: > Ser133  -  CCNB1 (human)

Site Information
sPsPMETsGCAPAEE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448445

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 7 ) , mass spectrometry ( 7 ) , mutation of modification site ( 2 , 3 , 5 , 6 , 7 ) , phospho-antibody ( 1 , 2 , 5 ) , western blotting ( 1 , 5 , 6 )
Disease tissue studied:
bone cancer ( 5 ) , leukemia ( 1 ) , chronic myelogenous leukemia ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PLK1 (human) ( 5 , 6 , 7 )
Kinases, in vitro:
PLK1 (human) ( 4 , 5 , 6 , 7 ) , PLK3 (human) ( 4 )

Downstream Regulation
Effects of modification on CCNB1:
intracellular localization ( 3 , 6 , 7 )
Induce interaction with:
DNA ( 2 )

References 

1

Poli A, et al. (2014) A novel DAG-dependent mechanism links PKCɑ and Cyclin B1 regulating cell cycle progression. Oncotarget 5, 11526-40
25362646   Curated Info

2

Santos SD, Wollman R, Meyer T, Ferrell JE (2012) Spatial positive feedback at the onset of mitosis. Cell 149, 1500-13
22726437   Curated Info

3

Jang CY, Coppinger JA, Yates JR, Fang G (2010) Phospho-regulation of DDA3 function in mitosis. Biochem Biophys Res Commun 393, 259-63
20117088   Curated Info

4

Iida M, Matsuda M, Komatani H (2008) Plk3 phosphorylates topoisomerase IIalpha at Thr(1342), a site that is not recognized by Plk1. Biochem J 411, 27-32
18062778   Curated Info

5

Jackman M, Lindon C, Nigg EA, Pines J (2003) Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat Cell Biol 5, 143-8
12524548   Curated Info

6

Yuan J, et al. (2002) Cooperative phosphorylation including the activity of polo-like kinase 1 regulates the subcellular localization of cyclin B1. Oncogene 21, 8282-92
12447691   Curated Info

7

Toyoshima-Morimoto F, et al. (2001) Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase. Nature 410, 215-20
11242082   Curated Info