Thr67
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Home > Phosphorylation Site Page: > Thr67  -  CDC25C (human)

Site Information
LsILsGGtPkRCLDL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448420

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 9 ) , [32P] bio-synthetic labeling ( 9 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 7 ) , mutation of modification site ( 6 ) , peptide sequencing ( 9 ) , phospho-antibody ( 6 ) , phosphoamino acid analysis ( 9 ) , phosphopeptide mapping ( 9 ) , western blotting ( 6 )
Disease tissue studied:
bone cancer ( 6 ) , breast cancer ( 2 ) , cervical cancer ( 5 ) , cervical adenocarcinoma ( 5 ) , leukemia ( 3 ) , acute myelogenous leukemia ( 3 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
CDK1 (human) ( 9 )
Treatments:
AZD1152 ( 4 ) , BI2536 ( 4 ) , nocodazole ( 5 ) , ZM447439 ( 4 )

Downstream Regulation
Effects of modification on CDC25C:
molecular association, regulation ( 8 ) , protein degradation ( 8 )
Effects of modification on biological processes:
cell adhesion, altered ( 9 ) , cell cycle regulation ( 6 , 9 )
Induce interaction with:
NEDD4 (mouse) ( 8 ) , PIN1 (human) ( 8 )

References 

1

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

2

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

3

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

4

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

5

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

6

Franckhauser C, et al. (2010) Distinct pools of cdc25C are phosphorylated on specific TP sites and differentially localized in human mitotic cells. PLoS One 5, e11798
20668692   Curated Info

7

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

8

Lu PJ, Zhou XZ, Shen M, Lu KP (1999) Function of WW domains as phosphoserine- or phosphothreonine-binding modules. Science 283, 1325-8
10037602   Curated Info

9

Strausfeld U, et al. (1994) Activation of p34cdc2 protein kinase by microinjection of human cdc25C into mammalian cells. Requirement for prior phosphorylation of cdc25C by p34cdc2 on sites phosphorylated at mitosis. J Biol Chem 269, 5989-6000
8119945   Curated Info

10

Izumi T, Maller JL (1993) Elimination of cdc2 phosphorylation sites in the cdc25 phosphatase blocks initiation of M-phase. Mol Biol Cell 4, 1337-50
7513216   Curated Info