Ser96
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Home > Phosphorylation Site Page: > Ser96  -  AR (human)

Site Information
QQQGEDGsPQAHRRG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448416

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 13 , 14 , 15 ) , electrophoretic mobility shift ( 13 ) , immunoassay ( 6 ) , immunoprecipitation ( 2 , 15 ) , mass spectrometry ( 3 , 4 , 5 , 8 , 9 , 10 , 13 , 14 ) , mutation of modification site ( 2 , 11 , 13 , 14 , 15 ) , phospho-antibody ( 2 , 6 , 7 , 11 , 12 ) , phosphoamino acid analysis ( 14 ) , phosphopeptide mapping ( 14 ) , western blotting ( 2 , 6 , 15 )
Disease tissue studied:
breast cancer ( 4 ) , breast ductal carcinoma ( 4 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 , 4 ) , lung cancer ( 8 ) , non-small cell lung cancer ( 8 ) , prostate cancer ( 6 ) , SBMA ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CDK2 (human) ( 2 )
Kinases, in vitro:
CDK2 (human) ( 2 )
Putative upstream phosphatases:
PPP2CA (human) ( 12 )
Treatments:
colforsin ( 2 ) , metribolone ( 11 , 13 , 14 ) , okadaic_acid ( 11 )

Downstream Regulation
Effects of modification on AR:
protein conformation ( 2 ) , protein stabilization ( 2 )
Effects of modification on biological processes:
transcription, altered ( 12 )

Disease / Diagnostics Relevance
Relevant diseases:
prostate cancer ( 6 , 14 ) , SBMA ( 2 )

References 

1

Ren QN, et al. (2021) Phosphorylation of Androgen Receptor by mTORC1 Promotes Liver Steatosis and Tumorigenesis. Hepatology
34435708   Curated Info

2

Polanco MJ, et al. (2016) Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Sci Transl Med 8, 370ra181
28003546   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

6

Willder JM, et al. (2013) Androgen receptor phosphorylation at serine 515 by Cdk1 predicts biochemical relapse in prostate cancer patients. Br J Cancer 108, 139-48
23321516   Curated Info

7

McCall P, et al. (2013) Androgen receptor phosphorylation at serine 308 and serine 791 predicts enhanced survival in castrate resistant prostate cancer patients. Int J Mol Sci 14, 16656-71
23945560   Curated Info

8

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

9

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

10

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

11

Yang CS, et al. (2007) Ligand binding to the androgen receptor induces conformational changes that regulate phosphatase interactions. Mol Cell Biol 27, 3390-404
17325038   Curated Info

12

Yang CS, et al. (2005) Simian virus 40 small t antigen mediates conformation-dependent transfer of protein phosphatase 2A onto the androgen receptor. Mol Cell Biol 25, 1298-308
15684382   Curated Info

13

Wong HY, et al. (2004) Phosphorylation of androgen receptor isoforms. Biochem J 383, 267-76
15239671   Curated Info

14

Gioeli D, et al. (2002) Androgen receptor phosphorylation. Regulation and identification of the phosphorylation sites. J Biol Chem 277, 29304-14
12015328   Curated Info

15

Zhou ZX, Kemppainen JA, Wilson EM (1995) Identification of three proline-directed phosphorylation sites in the human androgen receptor. Mol Endocrinol 9, 605-15
7565807   Curated Info