Ser310
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Home > Phosphorylation Site Page: > Ser310  -  AR (human)

Site Information
TEDTAEysPFKGGYT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448413

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 , 12 ) , flow cytometry ( 4 ) , immunoassay ( 7 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 2 , 3 , 6 , 12 , 13 ) , mutation of modification site ( 5 , 9 , 12 ) , peptide sequencing ( 13 ) , phospho-antibody ( 1 , 3 , 4 , 7 , 8 , 10 , 11 ) , phosphoamino acid analysis ( 12 ) , phosphopeptide mapping ( 12 , 13 ) , western blotting ( 1 , 3 , 4 , 7 )
Disease tissue studied:
breast cancer ( 6 ) , breast ductal carcinoma ( 6 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , prostate cancer ( 1 , 3 , 7 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 10 ) , breast ( 2 , 6 ) , COS (fibroblast) ( 9 , 11 , 12 ) , HEK293T (epithelial) ( 5 ) , LNCaP (prostate cell) ( 3 , 7 , 12 ) , LNCaP clone FGC (prostate cell) ( 1 ) , PC3 (prostate cell) ( 3 ) , prostate ( 8 ) , prostate cell ( 7 ) , SF9 ( 13 )

Upstream Regulation
Putative in vivo kinases:
CDK1 (human) ( 1 , 4 ) , CDK11B (human) ( 9 ) , CDK5 (human) ( 3 )
Kinases, in vitro:
CDK1 (human) ( 4 ) , CDK11B (human) ( 9 ) , CDK5 (human) ( 3 )
Putative upstream phosphatases:
PPP2CA (human) ( 11 )
Treatments:
androstanolone ( 1 , 4 , 5 ) , CAPE ( 1 ) , ischemia ( 6 ) , metribolone ( 10 , 11 , 12 ) , okadaic_acid ( 10 )

Downstream Regulation
Effects of modification on AR:
activity, inhibited ( 9 ) , molecular association, regulation ( 5 ) , protein stabilization ( 3 )
Effects of modification on biological processes:
cell growth, inhibited ( 9 ) , transcription, altered ( 11 ) , transcription, induced ( 1 , 3 ) , transcription, inhibited ( 4 , 5 , 9 )
Induce interaction with:
CREB3 iso3 (human) ( 5 )
Inhibit interaction with:
DNA ( 4 )

Disease / Diagnostics Relevance
Relevant diseases:
prostate cancer ( 7 , 8 , 12 )

References 

1

Kuo YY, et al. (2019) Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81 and Ser213. Cell Commun Signal 17, 100
31429764   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Lindqvist J, et al. (2015) Cyclin-dependent kinase 5 acts as a critical determinant of AKT-dependent proliferation and regulates differential gene expression by the androgen receptor in prostate cancer cells. Mol Biol Cell 26, 1971-84
25851605   Curated Info

4

Koryakina Y, Knudsen KE, Gioeli D (2015) Cell-cycle-dependent regulation of androgen receptor function. Endocr Relat Cancer 22, 249-64
25691442   Curated Info

5

Kim Y, Kim J, Jang SW, Ko J (2015) The role of sLZIP in cyclin D3-mediated negative regulation of androgen receptor transactivation and its involvement in prostate cancer. Oncogene 34, 226-36
24441043   Curated Info

6

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

7

Willder JM, et al. (2013) Androgen receptor phosphorylation at serine 515 by Cdk1 predicts biochemical relapse in prostate cancer patients. Br J Cancer 108, 139-48
23321516   Curated Info

8

McCall P, et al. (2013) Androgen receptor phosphorylation at serine 308 and serine 791 predicts enhanced survival in castrate resistant prostate cancer patients. Int J Mol Sci 14, 16656-71
23945560   Curated Info

9

Zong H, et al. (2007) Cyclin D3/CDK11p58 complex is involved in the repression of androgen receptor. Mol Cell Biol 27, 7125-42
17698582   Curated Info

10

Yang CS, et al. (2007) Ligand binding to the androgen receptor induces conformational changes that regulate phosphatase interactions. Mol Cell Biol 27, 3390-404
17325038   Curated Info

11

Yang CS, et al. (2005) Simian virus 40 small t antigen mediates conformation-dependent transfer of protein phosphatase 2A onto the androgen receptor. Mol Cell Biol 25, 1298-308
15684382   Curated Info

12

Gioeli D, et al. (2002) Androgen receptor phosphorylation. Regulation and identification of the phosphorylation sites. J Biol Chem 277, 29304-14
12015328   Curated Info

13

Zhu Z, Becklin RR, Desiderio DM, Dalton JT (2001) Identification of a novel phosphorylation site in human androgen receptor by mass spectrometry. Biochem Biophys Res Commun 284, 836-44
11396978   Curated Info