Tyr113
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Home > Phosphorylation Site Page: > Tyr113  -  SLP76 (human)

Site Information
ssFEEDDyESPNDDQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448218

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 2 ) , immunoassay ( 2 ) , immunoprecipitation ( 4 , 5 ) , mutation of modification site ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 7 , 8 , 9 ) , western blotting ( 1 , 2 , 4 , 8 , 9 , 10 )
Disease tissue studied:
leukemia ( 2 , 3 , 4 ) , T cell leukemia ( 2 , 3 , 4 ) , mastocytoma ( 4 )
Relevant cell line - cell type - tissue:
HEK293T (epithelial) ( 7 ) , J14.SY ( 2 ) , Jurkat (T lymphocyte) ( 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 ) , lymphocyte-spleen ( 5 ) , natural killer cell ( 4 ) , P116 (T lymphocyte) ( 9 ) , P815 (mast) ( 4 ) , S2 ( 1 , 4 ) , T lymphocyte ( 2 ) , thymocyte-thymus ( 5 )

Upstream Regulation
Regulatory protein:
CD244 (human) ( 4 ) , CD48 (human) ( 4 ) , DNAM-1 (human) ( 4 ) , FCGR3A (human) ( 4 ) , Fyn (human) ( 4 ) , KLRK1 (human) ( 4 ) , ULBP1 (human) ( 4 )
Putative in vivo kinases:
Syk (human) ( 1 , 7 ) , ZAP70 (human) ( 8 )
Kinases, in vitro:
Lck (human) ( 10 ) , ZAP70 (human) ( 10 )
Treatments:
antibody ( 2 , 4 , 5 , 7 , 10 ) , CAS_227449-73-2 ( 4 ) , CXCL12 ( 2 , 8 ) , piceatannol ( 4 ) , PP2 ( 4 ) , vanadate ( 7 )

Downstream Regulation
Effects of modification on SLP76:
molecular association, regulation ( 3 , 4 , 6 , 7 ) , phosphorylation ( 5 , 6 , 8 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 2 , 3 ) , signaling pathway regulation ( 4 ) , transcription, induced ( 2 , 9 )
Induce interaction with:
ITK (human) ( 7 ) , NCK1 (human) ( 3 , 7 ) , PIK3R1 (cow) ( 7 ) , VAV1 (human) ( 3 , 4 , 6 , 7 )

References 

1

Fasbender F, et al. (2017) Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes. Front Immunol 8, 789
28736554   Curated Info

2

Smith X, et al. (2013) The Chemokine CXCL12 Generates Costimulatory Signals in T Cells to Enhance Phosphorylation and Clustering of the Adaptor Protein SLP-76. Sci Signal 6, ra65
23901140   Curated Info

3

Pauker MH, et al. (2012) Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET. Sci Signal 5, rs3
22534133   Curated Info

4

Kim HS, Long EO (2012) Complementary Phosphorylation Sites in the Adaptor Protein SLP-76 Promote Synergistic Activation of Natural Killer Cells. Sci Signal 5, ra49
22786724   Curated Info

5

Sela M, et al. (2011) Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells. EMBO J 30, 3160-72
21725281   Curated Info

6

Beach D, et al. (2007) Dual role of SLP-76 in mediating T cell receptor-induced activation of phospholipase C-gamma1. J Biol Chem 282, 2937-46
17148460   Curated Info

7

Shim EK, et al. (2004) Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase. FEBS Lett 575, 35-40
15388330   Curated Info

8

Kremer KN, et al. (2003) Distinct role of ZAP-70 and Src homology 2 domain-containing leukocyte protein of 76 kDa in the prolonged activation of extracellular signal-regulated protein kinase by the stromal cell-derived factor-1 alpha/CXCL12 chemokine. J Immunol 171, 360-7
12817019   Curated Info

9

Epler JA, et al. (2000) Regulation of beta 1 integrin-mediated adhesion by T cell receptor signaling involves ZAP-70 but differs from signaling events that regulate transcriptional activity. J Immunol 165, 4941-9
11046020   Curated Info

10

Wardenburg JB, et al. (1996) Phosphorylation of SLP-76 by the ZAP-70 protein-tyrosine kinase is required for T-cell receptor function. J Biol Chem 271, 19641-4
8702662   Curated Info