Thr581
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Home > Phosphorylation Site Page: > Thr581  -  MSK1 (human)

Site Information
PDNQPLKtPCFTLHY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448217

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 7 ) , mutation of modification site ( 7 ) , peptide sequencing ( 7 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , phosphopeptide mapping ( 7 ) , western blotting ( 1 , 2 , 4 , 6 , 7 , 8 , 9 )
Disease tissue studied:
adrenal cancer ( 2 ) , pheochromocytoma ( 2 ) , hypopharyngeal cancer ( 1 ) , leukemia ( 9 ) , chronic myelogenous leukemia ( 9 ) , T cell leukemia ( 9 ) , psoriasis ( 5 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 3 ) , 293 (epithelial) ( 7 ) , bone marrow ( 2 ) , CNE1 ( 1 ) , keratinocyte-skin ( 5 ) , KT-1 (myeloid) ( 9 ) , MEF (fibroblast) [P38A (mouse), homozygous knockout] ( 9 ) , MOLT-4 (T lymphocyte) ( 9 ) , myocyte-heart ( 4 , 6 , 8 ) , neuron-'brain, hippocampus' ( 3 ) , PC-12 (chromaffin) ( 2 )

Upstream Regulation
Regulatory protein:
LMP1 (herpesvirus) ( 1 ) , TNF-R1 (human) ( 4 ) , TNF-R2 (human) ( 4 )
Putative in vivo kinases:
ERK1 (human) ( 7 ) , ERK2 (human) ( 7 ) , P38A (human) ( 5 , 7 )
Treatments:
8-Rp-cAMP ( 8 ) , AACOCF3 ( 6 ) , anti-TNFR1 ( 4 ) , anti-TNFR2 ( 4 ) , electrical_stimulation ( 3 ) , FGF9 ( 2 ) , H-89 ( 1 , 8 ) , IFN-alpha ( 9 ) , PD184352 ( 7 ) , PD98059 ( 1 ) , phenylephrine ( 8 ) , phorbol_ester ( 7 ) , Ro31-8220 ( 8 ) , SB203580 ( 7 ) , TNF ( 4 ) , UV ( 7 ) , zinterol ( 6 )

Downstream Regulation
Effects of modification on MSK1:
enzymatic activity, induced ( 5 , 6 , 7 ) , intracellular localization ( 6 )
Effects of modification on biological processes:
cell growth, induced ( 1 ) , cytoskeletal reorganization ( 3 ) , transcription, induced ( 1 )

References 

1

Li B, et al. (2015) Mitogen- and stress-activated Kinase 1 mediates Epstein-Barr virus latent membrane protein 1-promoted cell transformation in nasopharyngeal carcinoma through its induction of Fra-1 and c-Jun genes. BMC Cancer 15, 390
25958199   Curated Info

2

Lonic A, et al. (2013) Phosphorylation of Serine 779 in Fibroblast Growth Factor Receptor 1 and 2 by Protein Kinase C{epsilon} Regulates Ras/Mitogen-activated Protein Kinase Signaling and Neuronal Differentiation. J Biol Chem 288, 14874-85
23564461   Curated Info

3

Wittmann M, et al. (2009) Synaptic activity induces dramatic changes in the geometry of the cell nucleus: interplay between nuclear structure, histone H3 phosphorylation, and nuclear calcium signaling. J Neurosci 29, 14687-700
19940164   Curated Info

4

Defer N, Azroyan A, Pecker F, Pavoine C (2007) TNFR1 and TNFR2 signaling interplay in cardiac myocytes. J Biol Chem 282, 35564-73
17913704   Curated Info

5

Funding AT, Johansen C, Kragballe K, Iversen L (2007) Mitogen- and stress-activated protein kinase 2 and cyclic AMP response element binding protein are activated in lesional psoriatic epidermis. J Invest Dermatol 127, 2012-9
17429437   Curated Info

6

Ait-Mamar B, et al. (2005) The cytosolic phospholipase A2 pathway, a safeguard of beta2-adrenergic cardiac effects in rat. J Biol Chem 280, 18881-90
15728587   Curated Info

7

McCoy CE, et al. (2005) MSK1 activity is controlled by multiple phosphorylation sites. Biochem J 387, 507-17
15568999   Curated Info

8

Markou T, Hadzopoulou-Cladaras M, Lazou A (2004) Phenylephrine induces activation of CREB in adult rat cardiac myocytes through MSK1 and PKA signaling pathways. J Mol Cell Cardiol 37, 1001-11
15522277   Curated Info

9

Li Y, et al. (2004) Role of p38alpha Map kinase in Type I interferon signaling. J Biol Chem 279, 970-9
14578350   Curated Info