Tyr368

Site Information
STKMHGDytLTLRKG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448134

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 4 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 4 ) , peptide sequencing ( 4 ) , phosphoamino acid analysis ( 4 )
Disease tissue studied:	breast cancer ( 2 , 3 )
Relevant cell line - cell type - tissue:	COS (fibroblast) ( 4 ) , MCF-10A (breast cell) ( 2 , 3 )

Upstream Regulation
Kinases, in vitro:	INSR (human) ( 4 )

References
1	Farnsworth C (2007) CST Curation Set: 3376; Year: 2007; Biosample/Treatment: cell line, purified protein/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
2	Gu T (2005) CST Curation Set: 752; Year: 2005; Biosample/Treatment: cell line, MCF-10A/-; Disease: breast cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
3	Gu T (2005) CST Curation Set: 753; Year: 2005; Biosample/Treatment: cell line, MCF-10A/-; Disease: breast cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
4	Hayashi H, et al. (1993) The alpha-type 85-kDa subunit of phosphatidylinositol 3-kinase is phosphorylated at tyrosines 368, 580, and 607 by the insulin receptor. J Biol Chem 268, 7107-17 8385099 Curated Info