Ser960

Site Information
LGtPLRHsGstsPyL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3216486

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 )
Disease tissue studied:	anthrax infection ( 7 ) , brain cancer ( 5 ) , leukemia ( 4 ) , acute myelogenous leukemia ( 4 ) , neuroendocrine cancer ( 5 )
Relevant cell line - cell type - tissue:	3T3 (fibroblast) ( 3 ) , blood ( 4 ) , brain ( 5 ) , MC3T3-E1 (preosteoblast) ( 2 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 6 ) , MEF (fibroblast) ( 6 ) , spleen ( 7 )

Upstream Regulation
Regulatory protein:	CDC42 (mouse) ( 3 ) , KRas (mouse) ( 3 ) , PAK4 (mouse) ( 3 )

References
1	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
2	Williams GR, et al. (2016) Exploring G protein-coupled receptor signaling networks using SILAC-based phosphoproteomics. Methods 92, 36-50 26160508 Curated Info
3	Gnad F, et al. (2013) Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics. Mol Cell Proteomics 12, 2070-80 23608596 Curated Info
4	Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27 22802335 Curated Info
5	Guo A (2011) CST Curation Set: 12732; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: brain cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981 Curated Info
6	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
7	Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927 21189417 Curated Info