Thr348

Site Information
DTEFTSRtPRDsPGI SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447992

In vivo Characterization
Methods used to characterize site in vivo:	flow cytometry ( 9 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 3 , 4 , 5 , 6 ) , mutation of modification site ( 2 ) , phospho-antibody ( 1 , 2 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ) , western blotting ( 1 , 2 , 7 , 8 , 9 , 10 , 11 , 13 , 15 , 17 )
Disease tissue studied:	breast cancer ( 11 ) , neuroblastoma ( 5 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 3 ) , 'brain, cerebral cortex' ( 2 , 14 ) , 'brain, striatum' ( 18 ) , 'neuron, hippocampal'-brain [neurogranin (mouse)] ( 17 ) , 3T3-L1 (fibroblast) ( 1 ) , colon ( 8 ) , HC11 (epithelial) ( 10 ) , heart ( 12 ) , hepatocyte-liver ( 15 ) , JB (epithelial) ( 13 ) , macrophage-peritoneum ( 4 ) , MEF (fibroblast) ( 15 , 19 ) , Mel-Ab (melanocyte) ( 7 , 16 ) , N1E-115 (neuron) ( 5 ) , NMuMG (epithelial) ( 10 ) , T lymphocyte-lymph node ( 9 ) , T lymphocyte-spleen ( 6 ) , T47D (breast cell) ( 11 )

Upstream Regulation
Regulatory protein:	FMR1 (mouse) ( 2 ) , neurogranin (mouse) ( 17 )
Treatments:	antibody ( 9 ) , bacterial infection ( 8 ) , colforsin ( 17 ) , EGCG ( 13 ) , EGF ( 10 , 15 ) , insulin ( 3 , 15 ) , ischemia/reperfusion ( 12 ) , LPA ( 5 , 16 ) , LY294002 ( 9 ) , NP68 ( 9 ) , PD184352 ( 15 ) , phorbol_ester ( 17 ) , PP2 ( 9 ) , PRL ( 10 ) , rapamycin ( 9 , 15 ) , ravoxertinib ( 1 ) , SL327 ( 2 ) , sphingosine_1-phosphate ( 7 ) , TGF-beta ( 18 ) , Theaflavins ( 13 ) , TTX ( 14 ) , U0126 ( 9 ) , UV ( 13 , 19 ) , visual experience ( 14 )

Upstream Regulation

Regulatory protein:

FMR1 (mouse) ( 2 ) , neurogranin (mouse) ( 17 )

Treatments:

antibody ( 9 ) , bacterial infection ( 8 ) , colforsin ( 17 ) , EGCG ( 13 ) , EGF ( 10 , 15 ) , insulin ( 3 , 15 ) , ischemia/reperfusion ( 12 ) , LPA ( 5 , 16 ) , LY294002 ( 9 ) , NP68 ( 9 ) , PD184352 ( 15 ) , phorbol_ester ( 17 ) , PP2 ( 9 ) , PRL ( 10 ) , rapamycin ( 9 , 15 ) , ravoxertinib ( 1 ) , SL327 ( 2 ) , sphingosine_1-phosphate ( 7 ) , TGF-beta ( 18 ) , Theaflavins ( 13 ) , TTX ( 14 ) , U0126 ( 9 ) , UV ( 13 , 19 ) , visual experience ( 14 )

Downstream Regulation
Effects of modification on p90RSK:	enzymatic activity, induced ( 16 )

References
1	Duan X, et al. (2022) Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate. Biochem J 35594055 Curated Info
2	Sawicka K, et al. (2016) Elevated ERK/p90 ribosomal S6 kinase activity underlies audiogenic seizure susceptibility in fragile X mice. Proc Natl Acad Sci U S A 113, E6290-E6297 27663742 Curated Info
3	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
4	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
5	Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201 21454597 Curated Info
6	Navarro MN, et al. (2011) Phosphoproteomic analysis reveals an intrinsic pathway for the regulation of histone deacetylase 7 that controls the function of cytotoxic T lymphocytes. Nat Immunol 12, 352-61 21399638 Curated Info
7	Kim DS, et al. (2011) Sphingosine-1-phosphate decreases melanin synthesis via microphthalmia-associated transcription factor phosphorylation through the S1P3 receptor subtype. J Pharm Pharmacol 63, 409-16 21749389 Curated Info
8	Chandrakesan P, et al. (2010) Novel changes in NF-{kappa}B activity during progression and regression phases of hyperplasia: role of MEK, ERK, and p38. J Biol Chem 285, 33485-98 20710027 Curated Info
9	Salmond RJ, Emery J, Okkenhaug K, Zamoyska R (2009) MAPK, phosphatidylinositol 3-kinase, and mammalian target of rapamycin pathways converge at the level of ribosomal protein S6 phosphorylation to control metabolic signaling in CD8 T cells. J Immunol 183, 7388-97 19917692 Curated Info
10	Haines E, et al. (2009) Tyrosine phosphorylation of Grb2: role in prolactin/epidermal growth factor cross talk in mammary epithelial cell growth and differentiation. Mol Cell Biol 29, 2505-20 19273609 Curated Info
11	Vicent GP, et al. (2006) Induction of progesterone target genes requires activation of Erk and Msk kinases and phosphorylation of histone H3. Mol Cell 24, 367-81 17081988 Curated Info
12	Maekawa N, et al. (2006) Inhibiting p90 ribosomal S6 kinase prevents (Na+)-H+ exchanger-mediated cardiac ischemia-reperfusion injury. Circulation 113, 2516-23 16717153 Curated Info
13	Zykova TA, et al. (2005) The signal transduction networks required for phosphorylation of STAT1 at Ser727 in mouse epidermal JB6 cells in the UVB response and inhibitory mechanisms of tea polyphenols. Carcinogenesis 26, 331-42 15550455 Curated Info
14	Suzuki S, al-Noori S, Butt SA, Pham TA (2004) Regulation of the CREB signaling cascade in the visual cortex by visual experience and neuronal activity. J Comp Neurol 479, 70-83 15389611 Curated Info
15	Pende M, et al. (2004) S6K1(-/-)/S6K2(-/-) mice exhibit perinatal lethality and rapamycin-sensitive 5'-terminal oligopyrimidine mRNA translation and reveal a mitogen-activated protein kinase-dependent S6 kinase pathway. Mol Cell Biol 24, 3112-24 15060135 Curated Info
16	Kim DS, et al. (2004) Effects of lysophosphatidic acid on melanogenesis. Chem Phys Lipids 127, 199-206 14726002 Curated Info
17	Wu J, Li J, Huang KP, Huang FL (2002) Attenuation of protein kinase C and cAMP-dependent protein kinase signal transduction in the neurogranin knockout mouse. J Biol Chem 277, 19498-505 11912190 Curated Info
18	Zhu Y, et al. (2002) Transforming growth factor-beta 1 increases bad phosphorylation and protects neurons against damage. J Neurosci 22, 3898-909 12019309 Curated Info
19	Zhang Y, et al. (2001) UVA induces Ser381 phosphorylation of p90RSK/MAPKAP-K1 via ERK and JNK pathways. J Biol Chem 276, 14572-80 11278279 Curated Info