Tyr215

Site Information
QsRGsPHyFsPFRPY SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 460106
Associated spectra: 1 CST

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 )
Relevant cell line - cell type - tissue:	'fat, brown' ( 6 ) , heart ( 6 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 1 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 1 ) , HL-1 (myocyte) ( 1 ) , kidney ( 6 ) , liver ( 3 , 4 ) , liver [leptin (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 5 ) , mpkCCD (renal) ( 7 ) , pancreas ( 6 ) , spleen ( 6 ) , testis ( 6 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 )

Relevant cell line - cell type - tissue:

'fat, brown' ( 6 ) , heart ( 6 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 1 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 1 ) , HL-1 (myocyte) ( 1 ) , kidney ( 6 ) , liver ( 3 , 4 ) , liver [leptin (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 5 ) , mpkCCD (renal) ( 7 ) , pancreas ( 6 ) , spleen ( 6 ) , testis ( 6 )

References
1	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
2	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
3	Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86 23567750 Curated Info
4	Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83 23140645 Curated Info
5	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
6	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
7	Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7 20139300 Curated Info
8	Zhou J (2006) CST Curation Set: 1969; Year: 2006; Biosample1/Treatment/Isotope: tissue, brain/untreated/L, Biosample2/Treatment/Isotope: tissue, brain/untreated/H; Disease: -; SILAC: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: YXp[ST] Curated Info