Ser60
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Home > Phosphorylation Site Page: > Ser60  -  MAF1 (human)

Site Information
PHVLEALsPPQtsGL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3211917

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 13 , 14 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 15 , 16 ) , mutation of modification site ( 13 , 14 ) , western blotting ( 13 , 14 )
Disease tissue studied:
bone cancer ( 14 ) , osteosarcoma ( 14 ) , breast cancer ( 2 , 4 , 5 , 8 ) , breast ductal carcinoma ( 4 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , cervical cancer ( 15 ) , cervical adenocarcinoma ( 15 ) , leukemia ( 10 ) , acute myelogenous leukemia ( 10 ) , lung cancer ( 8 ) , non-small cell lung cancer ( 8 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 14 ) , 293E (epithelial) ( 11 ) , A549 (pulmonary) ( 6 ) , breast ( 1 , 4 ) , BT-20 (breast cell) ( 8 ) , BT-549 (breast cell) ( 8 ) , Calu 6 (pulmonary) ( 8 ) , H2009 (pulmonary) ( 8 ) , H2077 (pulmonary) ( 8 ) , H2887 (pulmonary) ( 8 ) , H322M (pulmonary) ( 8 ) , HCC1359 (pulmonary) ( 8 ) , HCC366 (pulmonary) ( 8 ) , HCC4006 (pulmonary) ( 8 ) , HCC78 (pulmonary) ( 8 ) , HeLa (cervical) [MAF1 (human), transfection] ( 13 ) , HeLa (cervical) ( 3 , 12 , 16 ) , HeLa S3 (cervical) ( 15 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 5 ) , HMLER ('stem, breast cancer') ( 5 ) , HOP62 (pulmonary) ( 8 ) , IMR90hTERT (fibroblast) [MAF1 (human), transfection] ( 13 ) , K562 (erythroid) ( 7 ) , KG-1 (myeloid) ( 10 ) , MCF-7 (breast cell) ( 2 ) , MDA-MB-231 (breast cell) ( 8 ) , MDA-MB-468 (breast cell) ( 8 ) , MEF (fibroblast) [MAF1 (human), transfection] ( 13 ) , MG63 (bone cell) ( 14 ) , NCI-H1395 (pulmonary) ( 8 ) , NCI-H1568 (pulmonary) ( 8 ) , NCI-H157 (pulmonary) ( 8 ) , NCI-H1648 (pulmonary) ( 8 ) , NCI-H1666 (pulmonary) ( 8 ) , NCI-H2030 (pulmonary) ( 8 ) , NCI-H322 (pulmonary) ( 8 ) , NCI-H647 (pulmonary) ( 8 )

Upstream Regulation
Regulatory protein:
mTOR (human) ( 14 ) , TBK1 (human) ( 6 )
Putative in vivo kinases:
mTOR (human) ( 11 )
Kinases, in vitro:
mTOR (human) ( 13 )
Treatments:
MMS ( 13 ) , nocodazole ( 15 ) , rapamycin ( 11 , 13 ) , serum_starvation ( 13 ) , temsirolimus ( 14 ) , Torin1 ( 11 , 13 ) , WYE132 ( 14 )

Downstream Regulation
Effects of modification on MAF1:
activity, inhibited ( 13 )
Effects of modification on biological processes:
transcription, induced ( 13 , 14 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

6

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

7

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

8

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

9

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

10

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

11

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

12

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

13

Michels AA, et al. (2010) mTORC1 directly phosphorylates and regulates human MAF1. Mol Cell Biol 30, 3749-57
20516213   Curated Info

14

Shor B, et al. (2010) Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and control of RNA polymerase III-dependent transcription in cancer cells. J Biol Chem 285, 15380-92
20233713   Curated Info

15

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

16

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

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