Ser31

Site Information
APsQLPLsGsRLKRR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3211092

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 3 , 4 , 6 , 7 , 8 , 9 , 10 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 2 )
Disease tissue studied:	breast cancer ( 1 ) , cervical cancer ( 2 , 9 ) , cervical adenocarcinoma ( 2 , 9 ) , lung cancer ( 3 ) , non-small cell lung cancer ( 6 ) , non-small cell lung adenocarcinoma ( 3 , 6 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 8 , 10 ) , HeLa S3 (cervical) ( 2 , 7 , 9 ) , Jurkat (T lymphocyte) ( 4 ) , lung ( 3 ) , NCI-H2228 (pulmonary) ( 6 )

Upstream Regulation
Kinases, in vitro:	CDK1 (human) ( 2 )
Treatments:	ZK-Thiazolidinone ( 7 )

References
1	Fan G, et al. (2021) The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation. Nat Commun 12, 20 33397932 Curated Info
2	Singh SA, et al. (2014) Co-regulation proteomics reveals substrates and mechanisms of APC/C-dependent degradation. EMBO J 33, 385-99 24510915 Curated Info
3	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
4	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
5	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
6	Rikova K (2013) CST Curation Set: 18502; Year: 2013; Biosample/Treatment: cell line, H2228/crizotinib, geldanamycin; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pSQ, p[ST]QG Antibodies Used to Purify Peptides prior to LCMS: Phospho-ATM/ATR Substrate (SQ) (D23H2/D69H5) Rabbit mAb Cat#: 9607 Phospho-(Ser/Thr) ATM/ATR Substrate (S/T*QG) (P-S/T2-100) Rabbit mAb Cat#: 6966 Curated Info
7	Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457 20860994 Curated Info
8	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
9	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
10	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info