Ser435
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Home > Phosphorylation Site Page: > Ser435  -  BUB1B (human)

Site Information
REAELLtsAEkRAEM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3207627

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 , 5 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 2 , 5 ) , phospho-antibody ( 5 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:
HeLa (cervical) ( 1 , 2 , 3 , 4 , 5 , 6 ) , HeLa S3 (cervical) ( 7 ) , HeLa_BI (cervical) ( 4 ) , HeLa_Hesp (cervical) ( 4 ) , K562 (erythroid) ( 3 )

Upstream Regulation
Putative in vivo kinases:
TTK (human) ( 5 )
Treatments:
AZ3146 ( 2 ) , BI2536 ( 2 ) , hesperadin ( 5 ) , nocodazole ( 5 ) , seliciclib ( 5 ) , SP600125 ( 2 ) , staurosporine ( 5 ) , ZM447439 ( 2 )

References 

1

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

2

Tipton AR, et al. (2013) Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex. J Biol Chem 288, 35149-58
24151075   Curated Info

3

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

4

Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12
22067460   Curated Info

5

Huang H, et al. (2008) Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit. J Cell Biol 183, 667-80
19015317   Curated Info

6

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

7

Steen JA, et al. (2008) Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A 105, 6069-74
18420821   Curated Info