Thr349
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Home > Phosphorylation Site Page: > Thr349  -  WDR44 (human)

Site Information
sNsGRELtDEEILAS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3198305

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 )
Disease tissue studied:
breast cancer ( 2 ) , cervical cancer ( 6 ) , cervical adenocarcinoma ( 6 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) [ADRB1 (human), no information, overexpresses human beta1-adrenergic (ß1AR- HEK293)] ( 10 ) , BT-549 (breast cell) ( 2 ) , Calu 6 (pulmonary) ( 2 ) , Flp-In T-Rex-293 (epithelial) ( 3 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 3 ) , H2009 (pulmonary) ( 2 ) , H2077 (pulmonary) ( 2 ) , H2887 (pulmonary) ( 2 ) , H322M (pulmonary) ( 2 ) , HCC1359 (pulmonary) ( 2 ) , HCC1937 (breast cell) ( 2 ) , HCC2279 (pulmonary) ( 2 ) , HCC366 (pulmonary) ( 2 ) , HCC4006 (pulmonary) ( 2 ) , HCC78 (pulmonary) ( 2 ) , HCC827 (pulmonary) ( 2 ) , HeLa (cervical) ( 5 , 7 , 8 , 9 ) , HeLa S3 (cervical) ( 6 ) , HUES-9 ('stem, embryonic') ( 4 ) , LCLC-103H (pulmonary) ( 2 ) , liver ( 1 ) , MCF-7 (breast cell) ( 2 ) , MDA-MB-231 (breast cell) ( 2 ) , NCI-H1395 (pulmonary) ( 2 ) , NCI-H1568 (pulmonary) ( 2 ) , NCI-H157 (pulmonary) ( 2 ) , NCI-H1648 (pulmonary) ( 2 ) , NCI-H1666 (pulmonary) ( 2 ) , NCI-H2030 (pulmonary) ( 2 ) , NCI-H2172 (pulmonary) ( 2 ) , NCI-H520 (squamous) ( 2 ) , NCI-H647 (pulmonary) ( 2 ) , PC9 (pulmonary) ( 2 )

References 

1

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

2

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

3

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

4

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

5

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

6

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

7

Zhou J (2009) CST Curation Set: 7765; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXT-P) (60D12F4) Rabbit mAb Cat#: 10002
Curated Info

8

Zhou J (2009) CST Curation Set: 7763; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXT-P) (60D12F4) Rabbit mAb Cat#: 10002
Curated Info

9

Zhou J (2009) CST Curation Set: 7762; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXT-P) (60D12F4) Rabbit mAb Cat#: 10002
Curated Info

10

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info