Ser412

Site Information
PEEEEIGsPEPMAAP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3196571

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 )
Disease tissue studied:	breast cancer ( 3 , 8 ) , luminal A breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , cervical cancer ( 12 ) , cervical adenocarcinoma ( 12 ) , leukemia ( 9 ) , acute myelogenous leukemia ( 9 ) , lung cancer ( 8 ) , non-small cell lung cancer ( 8 )
Relevant cell line - cell type - tissue:	breast ( 1 ) , BT-474 (breast cell) ( 3 ) , BT-549 (breast cell) ( 8 ) , Calu 6 (pulmonary) ( 8 ) , H2077 (pulmonary) ( 8 ) , H322M (pulmonary) ( 8 ) , HCC827 (pulmonary) ( 8 ) , HeLa (cervical) ( 4 , 7 , 11 , 14 , 16 ) , HeLa S3 (cervical) [PLK1 (human), knockdown, Tet-inducible PLK1 siRNA] ( 10 ) , HeLa S3 (cervical) ( 10 , 12 , 13 ) , Jurkat (T lymphocyte) ( 6 , 15 ) , K562 (erythroid) ( 7 ) , KG-1 (myeloid) ( 9 ) , LCLC-103H (pulmonary) ( 8 ) , LOU-NH91 (squamous) ( 8 ) , MCF-7 (breast cell) ( 3 ) , MDA-MB-231 (breast cell) ( 8 ) , NCI-H1648 (pulmonary) ( 8 ) , NCI-H460 (pulmonary) ( 8 )

Upstream Regulation
Treatments:	nocodazole ( 12 )

References
1	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
2	Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610 27184836 Curated Info
3	Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290 27362937 Curated Info
4	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
5	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
6	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
7	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
8	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
9	Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56 22115753 Curated Info
10	Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457 20860994 Curated Info
11	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
12	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
13	Malik R, et al. (2009) Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages. J Proteome Res 8, 4553-63 19691289 Curated Info
14	Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8 19276368 Curated Info
15	Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46 19690332 Curated Info
16	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info