Ser230
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Home > Phosphorylation Site Page: > Ser230  -  HSF1 (human)

Site Information
PkYSRQFsLEHVHGS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448072

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 13 , 14 ) , flow cytometry ( 5 ) , immunoprecipitation ( 2 , 5 ) , mass spectrometry ( 9 , 10 , 11 , 12 , 13 ) , microscopy-colocalization with upstream kinase ( 5 ) , mutation of modification site ( 2 , 3 , 4 , 5 ) , peptide sequencing ( 13 ) , phospho-antibody ( 2 , 5 , 14 ) , phosphoamino acid analysis ( 14 ) , phosphopeptide mapping ( 14 ) , western blotting ( 2 , 5 )
Disease tissue studied:
colorectal cancer ( 5 ) , colorectal carcinoma ( 5 ) , endometrial cancer ( 1 ) , endometrial adenocarcinoma ( 1 ) , ovarian cancer ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 , 5 ) , HCT116 (intestinal) ( 5 ) , HEC-1B (endometrial) ( 1 ) , HeLa (cervical) ( 2 , 3 , 13 , 14 ) , HTBoA (ovarian) ( 1 ) , Jurkat (T lymphocyte) ( 9 , 10 , 11 , 12 ) , K562 (erythroid) ( 14 ) , MCAS (ovarian) ( 1 ) , MEF (fibroblast) ( 3 ) , myocyte-heart ( 4 )

Upstream Regulation
Putative in vivo kinases:
DAPK1 (human) ( 5 )
Kinases, in vitro:
CAMK2A (human) ( 14 ) , DAPK1 (human) ( 5 )
Treatments:
DAPK_inhibitor ( 5 ) , heat_shock ( 14 ) , KN-62 ( 2 , 5 ) , KRIBB11 ( 2 ) , TNF ( 5 ) , virus infection ( 2 )

Downstream Regulation
Effects of modification on HSF1:
intracellular localization ( 2 )
Effects of modification on biological processes:
apoptosis, induced ( 5 ) , transcription, induced ( 2 , 5 , 8 ) , transcription, inhibited ( 3 )
Induce interaction with:
DNA ( 5 )

References 

1

Yasuda K, et al. (2017) Phosphorylation of HSF1 at serine 326 residue is related to the maintenance of gynecologic cancer stem cells through expression of HSP27. Oncotarget 8, 31540-31553
28415561   Curated Info

2

Qiu Y, et al. (2016) Hsp70-1: upregulation via selective phosphorylation of heat shock factor 1 during coxsackieviral infection and promotion of viral replication via the AU-rich element. Cell Mol Life Sci 73, 1067-84
26361762   Curated Info

3

BudzyƄski MA, Puustinen MC, Joutsen J, Sistonen L (2015) Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation. Mol Cell Biol 35, 2530-40
25963659   Curated Info

4

Meijering RA, et al. (2015) RhoA Activation Sensitizes Cells to Proteotoxic Stimuli by Abrogating the HSF1-Dependent Heat Shock Response. PLoS One 10, e0133553
26193369   Curated Info

5

Benderska N, et al. (2014) DAPK-HSF1 interaction as a positive-feedback mechanism stimulating TNF-induced apoptosis in colorectal cancer cells. J Cell Sci 127, 5273-87
25380824   Curated Info

6

Yoon T, et al. (2014) 2,4-Bis(4-hydroxybenzyl)phenol Inhibits Heat Shock Transcription Factor 1 and Sensitizes Lung Cancer Cells to Conventional Anticancer Modalities. J Nat Prod 77, 1123-1129
24746225   Curated Info

7

Park SJ, Sohn HY, Park SI (2013) TRAIL regulates collagen production through HSF1-dependent Hsp47 expression in activated hepatic stellate cells. Cell Signal 25, 1635-43
23587601   Curated Info

8

Yih LH, Hsu NC, Kuo HH, Wu YC (2012) Inhibition of the heat shock response by PI103 enhances the cytotoxicity of arsenic trioxide. Toxicol Sci 128, 126-36
22496356   Curated Info

9

Guo A (2012) CST Curation Set: 13866; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

10

Mulhern D (2012) CST Curation Set: 13758; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

11

Mulhern D (2012) CST Curation Set: 13759; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

12

Mulhern D (2012) CST Curation Set: 13760; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

13

Guettouche T, Boellmann F, Lane WS, Voellmy R (2005) Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress. BMC Biochem 6, 4
15760475   Curated Info

14

Holmberg CI, et al. (2001) Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1. EMBO J 20, 3800-10
11447121   Curated Info