Lys70

Site Information
EVRSDRDkFVIFLDV SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 459001

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 ) , mass spectrometry ( 2 , 3 , 4 ) , mass spectrometry (in vitro) ( 2 ) , modification-specific antibody ( 2 ) , mutation of modification site ( 2 ) , western blotting ( 2 )
Disease tissue studied:	liver cancer ( 1 ) , hepatocellular carcinoma ( 1 )
Relevant cell line - cell type - tissue:	CHO (fibroblast) ( 1 ) , epithelial-lens ( 1 ) , eye cell ( 4 ) , HLE (hepatic) ( 1 ) , lens ( 2 , 3 )

Downstream Regulation
Effects of modification on CRYA1:	activity, induced ( 1 , 2 ) , molecular association, regulation ( 2 ) , protein conformation ( 2 )
Effects of modification on biological processes:	apoptosis, inhibited ( 1 )

References
1	Nahomi RB, et al. (2013) Chaperone peptides of α-crystallin inhibit epithelial cell apoptosis, protein insolubilization, and opacification in experimental cataracts. J Biol Chem 288, 13022-35 23508955 Curated Info
2	Nagaraj RH, et al. (2012) Acetylation of αA-crystallin in the human lens: effects on structure and chaperone function. Biochim Biophys Acta 1822, 120-9 22120592 Curated Info
3	MacCoss MJ, et al. (2002) Shotgun identification of protein modifications from protein complexes and lens tissue. Proc Natl Acad Sci U S A 99, 7900-5 12060738 Curated Info
4	Lin PP, Barry RC, Smith DL, Smith JB (1998) In vivo acetylation identified at lysine 70 of human lens alphaA-crystallin. Protein Sci 7, 1451-7 9655350 Curated Info