Lys381
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Home > Acetylation Site Page: > Lys381  -  p53 (human)

Site Information
GQstsRHkkLMFktE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 458983

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 17 ) , mass spectrometry ( 12 , 16 , 26 ) , mutation of modification site ( 14 , 16 , 17 , 22 , 24 ) , phospho-antibody ( 5 ) , western blotting ( 5 , 17 )
Disease tissue studied:
bone cancer ( 17 ) , breast cancer ( 22 ) , colorectal cancer ( 5 , 16 ) , colorectal carcinoma ( 5 , 16 ) , leukemia ( 26 ) , acute myelogenous leukemia ( 26 ) , lung cancer ( 14 , 16 , 17 , 24 ) , non-small cell lung cancer ( 14 , 16 , 17 , 24 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Treatments:
MS275 ( 12 ) , nicotinamide ( 16 ) , trichostatin_A ( 7 , 16 , 17 ) , vorinostat ( 12 )

Downstream Regulation
Effects of modification on p53:
protein degradation ( 22 , 24 ) , ubiquitination ( 24 )
Effects of modification on biological processes:
apoptosis, induced ( 16 ) , cell growth, induced ( 22 ) , transcription, altered ( 14 , 16 )

References 

1

Zheng S, et al. (2017) Inhibiting p53 Acetylation Reduces Cancer Chemotoxicity. Cancer Res 77, 4342-4354
28655792   Curated Info

2

Ryu HW, et al. (2017) HDAC6 deacetylates p53 at lysines 381/382 and differentially coordinates p53-induced apoptosis. Cancer Lett 391, 162-171
28153791   Curated Info

3

Knyphausen P, et al. (2016) Insights into Lysine Deacetylation of Natively Folded Substrate Proteins by Sirtuins. J Biol Chem 291, 14677-94
27226597   Curated Info

4

Tong Q, et al. (2015) An Acetyl-Methyl Switch Drives a Conformational Change in p53. Structure 23, 322-31
25651062   Curated Info

5

Sen N, Kumari R, Singh MI, Das S (2013) HDAC5, a key component in temporal regulation of p53-mediated transactivation in response to genotoxic stress. Mol Cell 52, 406-20
24120667   Curated Info

6

Kim SS, Benchimol S (2013) HDAC5--a critical player in the p53 acetylation network. Mol Cell 52, 289-90
24207022   Curated Info

7

Brochier C, et al. (2013) Specific acetylation of p53 by HDAC inhibition prevents DNA damage-induced apoptosis in neurons. J Neurosci 33, 8621-32
23678107   Curated Info

8

Kim K, et al. (2012) Functional interplay between p53 acetylation and H1.2 phosphorylation in p53-regulated transcription. Oncogene 31, 4290-301
22249259   Curated Info

9

Muñoz-Fontela C, et al. (2011) Acetylation is indispensable for p53 antiviral activity. Cell Cycle 10, 3701-5
22033337   Curated Info

10

Seo SK, et al. (2011) Histone deacetylase inhibitors sensitize human non-small cell lung cancer cells to ionizing radiation through acetyl p53-mediated c-myc down-regulation. J Thorac Oncol 6, 1313-9
21642861   Curated Info

11

Debnath S, et al. (2011) Peptide-protein interactions suggest that acetylation of lysines 381 and 382 of p53 is important for positive coactivator 4-p53 interaction. J Biol Chem 286, 25076-87
21586571   Curated Info

12

Choudhary C, et al. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science 325, 834-40
19608861   Curated Info

13

Wu SY, Chiang CM (2009) Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding. EMBO J 28, 1246-59
19339993   Curated Info

14

Tian C, et al. (2009) KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis. Nat Cell Biol 11, 580-91
19377469   Curated Info

15

Itahana Y, Ke H, Zhang Y (2009) p53 Oligomerization is essential for its C-terminal lysine acetylation. J Biol Chem 284, 5158-64
19106109   Curated Info

16

Tang Y, et al. (2008) Acetylation is indispensable for p53 activation. Cell 133, 612-26
18485870   Curated Info

17

Li AG, et al. (2007) An acetylation switch in p53 mediates holo-TFIID recruitment. Mol Cell 28, 408-21
17996705   Curated Info

18

Kawaguchi Y, Ito A, Appella E, Yao TP (2006) Charge modification at multiple C-terminal lysine residues regulates p53 oligomerization and its nucleus-cytoplasm trafficking. J Biol Chem 281, 1394-400
16291740   Curated Info

19

Luo J, et al. (2004) Acetylation of p53 augments its site-specific DNA binding both in vitro and in vivo. Proc Natl Acad Sci U S A 101, 2259-64
14982997   Curated Info

20

Li M, Luo J, Brooks CL, Gu W (2002) Acetylation of p53 inhibits its ubiquitination by Mdm2. J Biol Chem 277, 50607-11
12421820   Curated Info

21

Ito A, et al. (2002) MDM2-HDAC1-mediated deacetylation of p53 is required for its degradation. EMBO J 21, 6236-45
12426395   Curated Info

22

Nakamura S, Roth JA, Mukhopadhyay T (2002) Multiple lysine mutations in the C-terminus of p53 make it resistant to degradation mediated by MDM2 but not by human papillomavirus E6 and induce growth inhibition in MDM2-overexpressing cells. Oncogene 21, 2605-10
11971195   Curated Info

23

Ito A, et al. (2001) p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2. EMBO J 20, 1331-40
11250899   Curated Info

24

Nakamura S, Roth JA, Mukhopadhyay T (2000) Multiple lysine mutations in the C-terminal domain of p53 interfere with MDM2-dependent protein degradation and ubiquitination. Mol Cell Biol 20, 9391-8
11094089   Curated Info

25

Luo J, et al. (2000) Deacetylation of p53 modulates its effect on cell growth and apoptosis. Nature 408, 377-81
11099047   Curated Info

26

Abraham J, Kelly J, Thibault P, Benchimol S (2000) Post-translational modification of p53 protein in response to ionizing radiation analyzed by mass spectrometry. J Mol Biol 295, 853-64
10656795   Curated Info

27

Gu W, Roeder RG (1997) Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain. Cell 90, 595-606
9288740   Curated Info