Thr538

Site Information
LGDAKtNtFCGTPDY SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447907

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 7 ) , mass spectrometry ( 4 ) , mutation of modification site ( 7 ) , phospho-antibody ( 2 , 3 , 5 , 6 , 7 ) , western blotting ( 2 , 3 , 5 , 7 )
Relevant cell line - cell type - tissue:	3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 6 ) , artery ( 5 ) , B lymphocyte-spleen ( 7 ) , brain ( 4 ) , HEK293T (epithelial) ( 3 ) , kidney ( 4 ) , lung ( 4 ) , MEF (fibroblast) ( 3 ) , T lymphocyte ( 2 )

Upstream Regulation
Treatments:	CCL21 ( 2 ) , insulin ( 5 ) , ionomycin ( 2 ) , linoleic_acid ( 6 ) , palmitate ( 5 ) , phorbol_ester ( 2 , 7 ) , thrombin ( 3 )

References
1	Loh JT, et al. (2023) DOK3 promotes atopic dermatitis by enabling the phosphatase PP4C to inhibit the T cell signaling mediator CARD11. Sci Signal 16, eadg5171 37906628 Curated Info
2	Cannon JL, et al. (2013) PKCθ regulates T cell motility via ezrin-radixin-moesin localization to the uropod. PLoS One 8, e78940 24250818 Curated Info
3	Hatziapostolou M, et al. (2011) Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal 4, ra55 21868363 Curated Info
4	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
5	Bakker W, et al. (2008) Protein kinase C theta activation induces insulin-mediated constriction of muscle resistance arteries. Diabetes 57, 706-13 18086904 Curated Info
6	Gao Z, et al. (2004) Inhibition of insulin sensitivity by free fatty acids requires activation of multiple serine kinases in 3T3-L1 adipocytes. Mol Endocrinol 18, 2024-34 15143153 Curated Info
7	Thebault S, Ochoa-Garay J (2004) Characterization of TCR-induced phosphorylation of PKCtheta in primary murine lymphocytes. Mol Immunol 40, 931-42 14725789 Curated Info