Tyr996
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Home > Phosphorylation Site Page: > Tyr996  -  VEGFR2 (human)

Site Information
EEAPEDLyKDFLTLE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447904

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , immunoassay ( 2 ) , immunoprecipitation ( 3 , 6 ) , mutation of modification site ( 3 , 6 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 ) , western blotting ( 1 , 3 , 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 , 6 ) , brain ( 5 ) , HUVEC (endothelial) ( 3 , 4 )

Upstream Regulation
Regulatory protein:
SHP-1 (human) ( 4 )
Putative in vivo kinases:
VEGFR2 (human) ( 6 )
Kinases, in vitro:
VEGFR2 (human) ( 6 , 7 )
Putative upstream phosphatases:
PTPRJ (human) ( 3 ) , SHP-1 (human) ( 4 )
Phosphatases, in vitro:
PTPRJ (human) ( 3 )
Treatments:
PP2 ( 4 ) , PP3 ( 4 ) , siRNA ( 4 ) , VEGF ( 3 , 4 , 6 )

Downstream Regulation
Effects of modification on VEGFR2:
enzymatic activity, induced ( 6 ) , molecular association, regulation ( 6 ) , receptor internalization, altered ( 6 )
Effects of modification on biological processes:
signaling pathway regulation ( 1 )
Induce interaction with:
PLCG1 (human) ( 6 )

Disease / Diagnostics Relevance
Relevant diseases:
HNSCC ( 2 )

References 

1

Kanthou C, et al. (2014) Tumour cells expressing single VEGF isoforms display distinct growth, survival and migration characteristics. PLoS One 9, e104015
25119572   Curated Info

2

Frederick MJ, et al. (2011) Phosphoproteomic analysis of signaling pathways in head and neck squamous cell carcinoma patient samples. Am J Pathol 178, 548-71
21281788   Curated Info

3

Chabot C, et al. (2009) New role for the protein tyrosine phosphatase DEP-1 in Akt activation and endothelial cell survival. Mol Cell Biol 29, 241-53
18936167   Curated Info

4

Bhattacharya R, et al. (2008) Src homology 2 (SH2) domain containing protein tyrosine phosphatase-1 (SHP-1) dephosphorylates VEGF Receptor-2 and attenuates endothelial DNA synthesis, but not migration*. J Mol Signal 3, 8
18377662   Curated Info

5

Schaefer LK, Ren Z, Fuller GN, Schaefer TS (2002) Constitutive activation of Stat3alpha in brain tumors: localization to tumor endothelial cells and activation by the endothelial tyrosine kinase receptor (VEGFR-2). Oncogene 21, 2058-65
11960378   Curated Info

6

Dougher M, Terman BI (1999) Autophosphorylation of KDR in the kinase domain is required for maximal VEGF-stimulated kinase activity and receptor internalization. Oncogene 18, 1619-27
10102632   Curated Info

7

Dougher-Vermazen M, Hulmes JD, Böhlen P, Terman BI (1994) Biological activity and phosphorylation sites of the bacterially expressed cytosolic domain of the KDR VEGF-receptor. Biochem Biophys Res Commun 205, 728-38
7999104   Curated Info