Tyr1175
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Home > Phosphorylation Site Page: > Tyr1175  -  VEGFR2 (human)

Site Information
AQQDGKDyIVLPISE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447901

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 8 ) , immunoassay ( 8 ) , immunoprecipitation ( 8 , 11 , 13 , 15 ) , mutation of modification site ( 8 , 11 , 13 , 14 , 15 , 19 , 20 , 21 , 22 , 23 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 21 , 23 ) , western blotting ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 19 )
Relevant cell line - cell type - tissue:
293 (epithelial) [VEGFR2 (human)] ( 16 ) , 293 (epithelial) ( 11 , 13 , 23 ) , BAEC (endothelial) ( 14 ) , BAE ( 13 , 19 ) , COS (fibroblast) ( 14 ) , COS7 (fibroblast) ( 8 ) , HAEC (endothelial) ( 16 ) , HEK293T (epithelial) ( 19 ) , HMVEC (endothelial) ( 13 ) , HUVEC (endothelial) ( 1 , 3 , 4 , 5 , 7 , 8 , 10 , 11 , 12 , 13 , 19 , 21 ) , microvessel endothelial ( 9 ) , MSS31 (endothelial) ( 21 ) , PAE (endothelial) ( 15 , 17 , 18 , 20 , 22 , 23 ) , TIME (endothelial) ( 18 )

Upstream Regulation
Regulatory protein:
PDCD6 (human) ( 7 ) , PPP2CA (human) ( 16 ) , PTPN13 (human) ( 5 ) , SHP-1 (human) ( 12 )
Putative in vivo kinases:
Src (human) ( 13 ) , VEGFR2 (human) ( 21 )
Kinases, in vitro:
VEGFR2 (human) ( 21 )
Putative upstream phosphatases:
PTPRJ (human) ( 11 ) , SHP-1 (human) ( 12 )
Phosphatases, in vitro:
PTPRJ (human) ( 11 )
Treatments:
diabetes ( 1 ) , IL-1b ( 9 ) , M-CSF ( 13 , 17 ) , mechanical stress ( 9 ) , Ox-PAPC ( 16 ) , PP2 ( 12 ) , PP3 ( 12 ) , siRNA ( 12 ) , SU6656 ( 13 ) , TNF ( 19 ) , VEGF ( 4 , 5 , 7 , 10 , 11 , 12 , 13 , 16 , 18 , 19 , 21 , 23 ) , VEGF_Ax ( 3 )

Downstream Regulation
Effects of modification on VEGFR2:
enzymatic activity, induced ( 16 ) , molecular association, regulation ( 8 , 18 , 20 , 21 , 23 ) , phosphorylation ( 8 , 14 , 19 )
Effects of modification on biological processes:
carcinogenesis, induced ( 7 ) , cell growth, altered ( 21 ) , cell growth, induced ( 7 ) , cell motility, induced ( 8 ) , signaling pathway regulation ( 6 , 7 , 22 ) , transcription, altered ( 16 )
Induce interaction with:
NRP1 (human) ( 8 ) , PLCG1 (human) ( 20 , 21 ) , SHB (human) ( 18 ) , SHC1 (human) ( 20 ) , SHC2 (human) ( 20 , 23 )

References 

1

Troncoso F, et al. (2017) Gestational diabetes mellitus is associated with increased pro-migratory activation of vascular endothelial growth factor receptor 2 and reduced expression of vascular endothelial growth factor receptor 1. PLoS One 12, e0182509
28817576   Curated Info

2

Kim GY, et al. (2016) Coronin 1B serine 2 phosphorylation by p38α is critical for vascular endothelial growth factor-induced migration of human umbilical vein endothelial cells. Cell Signal 28, 1817-1825
27592029   Curated Info

3

Xin H, Zhong C, Nudleman E, Ferrara N (2016) Evidence for Pro-angiogenic Functions of VEGF-Ax. Cell 167, 275-284.e6
27662093   Curated Info

4

Colladel R, et al. (2016) MULTIMERIN2 binds VEGF-A primarily via the carbohydrate chains exerting an angiostatic function and impairing tumor growth. Oncotarget 7, 2022-37
26655500   Curated Info

5

Yamana S, et al. (2015) The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation. PLoS One 10, e0124259
25893857   Curated Info

6

Kanthou C, et al. (2014) Tumour cells expressing single VEGF isoforms display distinct growth, survival and migration characteristics. PLoS One 9, e104015
25119572   Curated Info

7

Rho SB, et al. (2012) Programmed cell death 6 (PDCD6) inhibits angiogenesis through PI3K/mTOR/p70S6K pathway by interacting of VEGFR-2. Cell Signal 24, 131-9
21893193   Curated Info

8

Herzog B, et al. (2011) VEGF binding to NRP1 is essential for VEGF stimulation of endothelial cell migration, complex formation between NRP1 and VEGFR2, and signaling via FAK Tyr407 phosphorylation. Mol Biol Cell 22, 2766-76
21653826   Curated Info

9

Liu J, Agarwal S (2010) Mechanical signals activate vascular endothelial growth factor receptor-2 to upregulate endothelial cell proliferation during inflammation. J Immunol 185, 1215-21
20548028   Curated Info

10

Chen TT, et al. (2010) Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188, 595-609
20176926   Curated Info

11

Chabot C, et al. (2009) New role for the protein tyrosine phosphatase DEP-1 in Akt activation and endothelial cell survival. Mol Cell Biol 29, 241-53
18936167   Curated Info

12

Bhattacharya R, et al. (2008) Src homology 2 (SH2) domain containing protein tyrosine phosphatase-1 (SHP-1) dephosphorylates VEGF Receptor-2 and attenuates endothelial DNA synthesis, but not migration*. J Mol Signal 3, 8
18377662   Curated Info

13

Meyer RD, Sacks DB, Rahimi N (2008) IQGAP1-dependent signaling pathway regulates endothelial cell proliferation and angiogenesis. PLoS One 3, e3848
19050761   Curated Info

14

Blanes MG, Oubaha M, Rautureau Y, Gratton JP (2007) Phosphorylation of tyrosine 801 of vascular endothelial growth factor receptor-2 is necessary for Akt-dependent endothelial nitric-oxide synthase activation and nitric oxide release from endothelial cells. J Biol Chem 282, 10660-9
17303569   Curated Info

15

Singh AJ, et al. (2007) A critical role for the E3-ligase activity of c-Cbl in VEGFR-2-mediated PLCgamma1 activation and angiogenesis. Proc Natl Acad Sci U S A 104, 5413-8
17372230   Curated Info

16

Zimman A, et al. (2007) Vascular endothelial growth factor receptor 2 plays a role in the activation of aortic endothelial cells by oxidized phospholipids. Arterioscler Thromb Vasc Biol 27, 332-8
17110601   Curated Info

17

Meyer RD, Mohammadi M, Rahimi N (2006) A single amino acid substitution in the activation loop defines the decoy characteristic of VEGFR-1/FLT-1. J Biol Chem 281, 867-75
16286478   Curated Info

18

Holmqvist K, et al. (2004) The adaptor protein shb binds to tyrosine 1175 in vascular endothelial growth factor (VEGF) receptor-2 and regulates VEGF-dependent cellular migration. J Biol Chem 279, 22267-75
15026417   Curated Info

19

Zhang R, et al. (2003) Etk/Bmx transactivates vascular endothelial growth factor 2 and recruits phosphatidylinositol 3-kinase to mediate the tumor necrosis factor-induced angiogenic pathway. J Biol Chem 278, 51267-76
14532277   Curated Info

20

Ratcliffe KE, et al. (2002) Sck is expressed in endothelial cells and participates in vascular endothelial growth factor-induced signaling. Oncogene 21, 6307-16
12214271   Curated Info

21

Takahashi T, Yamaguchi S, Chida K, Shibuya M (2001) A single autophosphorylation site on KDR/Flk-1 is essential for VEGF-A-dependent activation of PLC-gamma and DNA synthesis in vascular endothelial cells. EMBO J 20, 2768-78
11387210   Curated Info

22

Knight EL, Warner AJ, Maxwell A, Prigent SA (2000) Chimeric VEGFRs are activated by a small-molecule dimerizer and mediate downstream signalling cascades in endothelial cells. Oncogene 19, 5398-405
11103941   Curated Info

23

Warner AJ, et al. (2000) The Shc-related adaptor protein, Sck, forms a complex with the vascular-endothelial-growth-factor receptor KDR in transfected cells. Biochem J 347, 501-9
10749680   Curated Info