Ser383
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Home > Phosphorylation Site Page: > Ser383  -  ELK1 (human)

Site Information
IHFWSTLsPIAPRsP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447872

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 24 ) , [32P] bio-synthetic labeling ( 24 , 27 ) , electrophoretic mobility shift ( 17 ) , flow cytometry ( 6 ) , immunoassay ( 11 ) , immunoprecipitation ( 7 , 13 ) , microscopy-colocalization with upstream kinase ( 3 ) , mutation of modification site ( 3 , 11 , 15 , 22 , 24 , 27 ) , phospho-antibody ( 2 , 3 , 4 , 6 , 7 , 8 , 9 , 11 , 13 , 14 , 15 , 16 , 17 , 19 , 20 , 21 , 23 , 25 , 26 ) , phosphopeptide mapping ( 27 ) , western blotting ( 2 , 3 , 4 , 6 , 7 , 8 , 9 , 13 , 14 , 15 , 16 , 20 )
Disease tissue studied:
brain cancer ( 3 ) , glioblastoma ( 3 ) , glioma ( 3 ) , breast cancer ( 2 , 6 , 7 , 9 ) , leukemia ( 8 ) , acute myelogenous leukemia ( 8 ) , lung cancer ( 13 ) , non-small cell lung cancer ( 13 ) , neuroblastoma ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
ARRB1 (human) ( 13 ) , ARRB2 (human) ( 13 ) , CK2A1 (human) ( 4 ) , DAGLBETA (human) ( 13 ) , DUSP1 (human) ( 14 ) , ERK1 (human) ( 24 ) , IL17RD (human) ( 13 ) , IQGAP1 (human) ( 13 ) , KSR (human) ( 13 ) , P38A (human) ( 24 ) , PXN iso2 (human) ( 13 ) , TAO2 (human) ( 16 ) , UBC9 (human) ( 15 )
Putative in vivo kinases:
ERK1 (human) ( 27 ) , P38A (human) ( 25 )
Kinases, in vitro:
ERK1 (human) ( 8 , 18 , 24 , 27 ) , ERK1 (mouse) ( 19 ) , ERK2 (human) ( 8 ) , ERK2 (mouse) ( 19 ) , MSK1 (human) ( 10 ) , P38A (human) ( 24 )
Treatments:
2-deoxyglucose ( 12 ) , 3-O-methylglucose ( 19 ) , anisomycin ( 17 ) , apigenin ( 8 ) , arsenite ( 17 ) , depolarization ( 19 ) , diazoxide ( 19 ) , EGF ( 6 , 7 , 9 , 13 , 19 , 21 , 26 ) , estradiol ( 22 ) , faslodex ( 22 ) , fibronectin ( 21 ) , glucose ( 19 ) , grifolin ( 2 ) , IFN-gamma ( 8 ) , LY294002 ( 8 ) , mannitol ( 19 ) , mannose ( 19 ) , PD98059 ( 2 , 8 , 16 , 17 , 19 , 22 ) , phorbol_ester ( 15 , 23 ) , rottlerin ( 8 ) , SB203580 ( 17 ) , serum ( 14 , 23 ) , SP600125 ( 8 ) , TBB ( 4 ) , TGF-beta ( 22 ) , tolbutamide ( 19 ) , tPA ( 4 ) , U0126 ( 4 , 6 , 7 , 9 , 15 ) , wortmannin ( 9 , 19 )

Downstream Regulation
Effects of modification on ELK1:
activity, induced ( 27 ) , intracellular localization ( 3 ) , molecular association, regulation ( 7 ) , protein conformation ( 27 )
Effects of modification on biological processes:
apoptosis, altered ( 6 ) , transcription, altered ( 7 , 22 ) , transcription, induced ( 4 , 6 , 15 , 24 , 27 )
Induce interaction with:
AurA (human) ( 3 ) , DNA ( 7 )

References 

1

Zhu G, Herlyn M, Yang X (2021) TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination. Nat Cell Biol 23, 978-991
34497368   Curated Info

2

Luo X, et al. (2015) Grifolin directly targets ERK1/2 to epigenetically suppress cancer cell metastasis. Oncotarget 6, 42704-16
26516701   Curated Info

3

Demir O, Kurnaz IA (2013) Phospho-Ser383-Elk-1 is localized to the mitotic spindles during cell cycle and interacts with mitotic kinase Aurora-A. Cell Biochem Funct 31, 591-8
23322625   Curated Info

4

Plotnikov A, et al. (2011) Nuclear extracellular signal-regulated kinase 1 and 2 translocation is mediated by casein kinase 2 and accelerated by autophosphorylation. Mol Cell Biol 31, 3515-30
21730285   Curated Info

5

Clippinger AJ, Maguire TG, Alwine JC (2011) Human cytomegalovirus infection maintains mTOR activity and its perinuclear localization during amino acid deprivation. J Virol 85, 9369-76
21734039   Curated Info

6

Booy EP, Henson ES, Gibson SB (2011) Epidermal growth factor regulates Mcl-1 expression through the MAPK-Elk-1 signalling pathway contributing to cell survival in breast cancer. Oncogene 30, 2367-78
21258408   Curated Info

7

Wyrzykowska P, et al. (2010) Epidermal growth factor regulates PAI-1 expression via activation of the transcription factor Elk-1. Biochim Biophys Acta 1799, 616-21
20727996   Curated Info

8

Li N, et al. (2010) ERK is integral to the IFN-gamma-mediated activation of STAT1, the expression of key genes implicated in atherosclerosis, and the uptake of modified lipoproteins by human macrophages. J Immunol 185, 3041-8
20675591   Curated Info

9

Aksamitiene E, et al. (2010) PI3K/Akt-sensitive MEK-independent compensatory circuit of ERK activation in ER-positive PI3K-mutant T47D breast cancer cells. Cell Signal 22, 1369-78
20471474   Curated Info

10

Shimada M, Nakadai T, Fukuda A, Hisatake K (2010) cAMP-response element-binding protein (CREB) controls MSK1-mediated phosphorylation of histone H3 at the c-fos promoter in vitro. J Biol Chem 285, 9390-401
20089855   Curated Info

11

Sharma A, et al. (2010) A neurotoxic phosphoform of Elk-1 associates with inclusions from multiple neurodegenerative diseases. PLoS One 5, e9002
20126313   Curated Info

12

Zhong D, et al. (2009) The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathways through IGF1R. J Biol Chem 284, 23225-33
19574224   Curated Info

13

Casar B, Pinto A, Crespo P (2008) Essential role of ERK dimers in the activation of cytoplasmic but not nuclear substrates by ERK-scaffold complexes. Mol Cell 31, 708-21
18775330   Curated Info

14

Wu JJ, Zhang L, Bennett AM (2005) The noncatalytic amino terminus of mitogen-activated protein kinase phosphatase 1 directs nuclear targeting and serum response element transcriptional regulation. Mol Cell Biol 25, 4792-803
15899879   Curated Info

15

Yang SH, Jaffray E, Hay RT, Sharrocks AD (2003) Dynamic interplay of the SUMO and ERK pathways in regulating Elk-1 transcriptional activity. Mol Cell 12, 63-74
12887893   Curated Info

16

Chen Z, et al. (2003) TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors. J Biol Chem 278, 22278-83
12665513   Curated Info

17

B├ębien M, et al. (2003) Immediate-early gene induction by the stresses anisomycin and arsenite in human osteosarcoma cells involves MAPK cascade signaling to Elk-1, CREB and SRF. Oncogene 22, 1836-47
12660819   Curated Info

18

She QB, Ma WY, Dong Z (2002) Role of MAP kinases in UVB-induced phosphorylation of p53 at serine 20. Oncogene 21, 1580-9
11896587   Curated Info

19

Bernal-Mizrachi E, et al. (2001) Activation of Elk-1, an Ets transcription factor, by glucose and EGF treatment of insulinoma cells. Am J Physiol Endocrinol Metab 281, E1286-99
11701445   Curated Info

20

Xu Be, Stippec S, Robinson FL, Cobb MH (2001) Hydrophobic as well as charged residues in both MEK1 and ERK2 are important for their proper docking. J Biol Chem 276, 26509-15
11352917   Curated Info

21

Aplin AE, Stewart SA, Assoian RK, Juliano RL (2001) Integrin-mediated adhesion regulates ERK nuclear translocation and phosphorylation of Elk-1. J Cell Biol 153, 273-82
11309409   Curated Info

22

Duan R, Xie W, Burghardt RC, Safe S (2001) Estrogen receptor-mediated activation of the serum response element in MCF-7 cells through MAPK-dependent phosphorylation of Elk-1. J Biol Chem 276, 11590-8
11145955   Curated Info

23

Cruzalegui FH, Cano E, Treisman R (1999) ERK activation induces phosphorylation of Elk-1 at multiple S/T-P motifs to high stoichiometry. Oncogene 18, 7948-57
10637505   Curated Info

24

Janknecht R, Hunter T (1997) Convergence of MAP kinase pathways on the ternary complex factor Sap-1a. EMBO J 16, 1620-7
9130707   Curated Info

25

Tan Y, et al. (1996) FGF and stress regulate CREB and ATF-1 via a pathway involving p38 MAP kinase and MAPKAP kinase-2. EMBO J 15, 4629-42
8887554   Curated Info

26

Xing J, Ginty DD, Greenberg ME (1996) Coupling of the RAS-MAPK pathway to gene activation by RSK2, a growth factor-regulated CREB kinase. Science 273, 959-63
8688081   Curated Info

27

Janknecht R, Ernst WH, Pingoud V, Nordheim A (1993) Activation of ternary complex factor Elk-1 by MAP kinases. EMBO J 12, 5097-104
8262053   Curated Info