Lys422
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Home > Sumoylation Site Page: > Lys422  -  hnRNP K (human)

Site Information
HEsGAsIkIDEPLEG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2894422

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 6 ) , electrophoretic mobility shift ( 5 ) , mass spectrometry ( 2 , 4 , 6 ) , mass spectrometry (in vitro) ( 6 ) , mutation of modification site ( 5 ) , western blotting ( 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 6 ) , 293N3S (epithelial) ( 4 ) , HEK293T (epithelial) ( 5 ) , HeLa (cervical) [SUMO2 (human), transfection, SUMO2 K0 Q87R (lysine-deficient SUMO2, with Q87R to create convenient Trypsin cleavage site)] ( 2 ) , HeLa (cervical) ( 5 )

Upstream Regulation
Regulatory protein:
ATR (human) ( 5 )
Treatments:
adriamycin ( 5 ) , caffeine ( 5 ) , ionizing_radiation ( 5 ) , siRNA ( 5 ) , UV ( 5 )

Downstream Regulation
Effects of modification on hnRNP K:
intracellular localization ( 5 ) , molecular association, regulation ( 5 ) , protein stabilization ( 5 ) , ubiquitination ( 5 )
Effects of modification on biological processes:
cell cycle regulation ( 5 ) , transcription, induced ( 5 )
Induce interaction with:
DNA ( 5 ) , p53 (human) ( 5 )
Inhibit interaction with:
MDM2 (human) ( 5 )

References 

1

Lumpkin RJ, et al. (2017) Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. Nat Commun 8, 1171
29079793   Curated Info

2

Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36
25218447   Curated Info

3

Impens F, Radoshevich L, Cossart P, Ribet D (2014) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli. Proc Natl Acad Sci U S A 111, 12432-7
25114211   Curated Info

4

Tammsalu T, et al. (2014) Proteome-wide identification of SUMO2 modification sites. Sci Signal 7, rs2
24782567   Curated Info

5

Lee SW, et al. (2012) SUMOylation of hnRNP-K is required for p53-mediated cell-cycle arrest in response to DNA damage. EMBO J 31, 4441-52
23092970   Curated Info

6

Li T, et al. (2004) Sumoylation of heterogeneous nuclear ribonucleoproteins, zinc finger proteins, and nuclear pore complex proteins: a proteomic analysis. Proc Natl Acad Sci U S A 101, 8551-6
15161980   Curated Info

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