Tyr114
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Home > Phosphorylation Site Page: > Tyr114  -  FHIT (human)

Site Information
FHRNDsIyEELQKHD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447802

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 5 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry (in vitro) ( 4 ) , mutation of modification site ( 1 , 2 , 3 , 5 ) , phospho-antibody ( 2 , 5 ) , western blotting ( 1 , 2 , 5 )
Disease tissue studied:
breast cancer ( 2 ) , lung cancer ( 1 , 3 ) , non-small cell lung cancer ( 1 , 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Src (human) ( 2 )
Putative in vivo kinases:
Src (human) ( 5 )
Kinases, in vitro:
Src (human) ( 5 )
Treatments:
lactacystin ( 2 )

Downstream Regulation
Effects of modification on FHIT:
activity, inhibited ( 3 ) , enzymatic activity, inhibited ( 4 ) , intracellular localization ( 1 ) , protein degradation ( 2 )
Effects of modification on biological processes:
apoptosis, altered ( 1 ) , apoptosis, induced ( 3 ) , cell cycle regulation ( 1 ) , cell growth, altered ( 1 )
Induce interaction with:
FDXR (human) ( 1 ) , HSP60 (human) ( 1 )

References 

1

Pichiorri F, et al. (2009) Correlation of fragile histidine triad (Fhit) protein structural features with effector interactions and biological functions. J Biol Chem 284, 1040-9
19004824   Curated Info

2

Bianchi F, et al. (2006) FHIT-proteasome degradation caused by mitogenic stimulation of the EGF receptor family in cancer cells. Proc Natl Acad Sci U S A 103, 18981-6
17142325   Curated Info

3

Semba S, et al. (2006) Fhit modulation of the Akt-survivin pathway in lung cancer cells: Fhit-tyrosine 114 (Y114) is essential. Oncogene 25, 2860-72
16407838   Curated Info

4

Garrison PN, et al. (2005) Phosphorylation of the human Fhit tumor suppressor on tyrosine 114 in Escherichia coli and unexpected steady state kinetics of the phosphorylated forms. Biochemistry 44, 6286-92
15835917   Curated Info

5

Pekarsky Y, et al. (2004) Fhit is a physiological target of the protein kinase Src. Proc Natl Acad Sci U S A 101, 3775-9
15007172   Curated Info