Tyr114

Site Information
FHRNDsIyEELQKHD SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447802

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 5 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry (in vitro) ( 4 ) , mutation of modification site ( 1 , 2 , 3 , 5 ) , phospho-antibody ( 2 , 5 ) , western blotting ( 1 , 2 , 5 )
Disease tissue studied:	breast cancer ( 2 ) , lung cancer ( 1 , 3 ) , non-small cell lung cancer ( 1 , 3 )
Relevant cell line - cell type - tissue:	'liver, fetal' ( 5 ) , 293 (epithelial) ( 2 , 5 ) , A549 (pulmonary) ( 3 ) , kidney ( 5 ) , MDA-MB-453 (breast cell) ( 2 ) , MKN-74 (gastric) ( 1 ) , NCI-H1299 (pulmonary) ( 1 , 3 ) , SKBr3 (breast cell) ( 2 )

Downstream Regulation
Effects of modification on FHIT:	activity, inhibited ( 3 ) , enzymatic activity, inhibited ( 4 ) , intracellular localization ( 1 ) , protein degradation ( 2 )
Effects of modification on biological processes:	apoptosis, altered ( 1 ) , apoptosis, induced ( 3 ) , cell cycle regulation ( 1 ) , cell growth, altered ( 1 )
Induce interaction with:	FDXR (human) ( 1 ) , HSP60 (human) ( 1 )

References
1	Pichiorri F, et al. (2009) Correlation of fragile histidine triad (Fhit) protein structural features with effector interactions and biological functions. J Biol Chem 284, 1040-9 19004824 Curated Info
2	Bianchi F, et al. (2006) FHIT-proteasome degradation caused by mitogenic stimulation of the EGF receptor family in cancer cells. Proc Natl Acad Sci U S A 103, 18981-6 17142325 Curated Info
3	Semba S, et al. (2006) Fhit modulation of the Akt-survivin pathway in lung cancer cells: Fhit-tyrosine 114 (Y114) is essential. Oncogene 25, 2860-72 16407838 Curated Info
4	Garrison PN, et al. (2005) Phosphorylation of the human Fhit tumor suppressor on tyrosine 114 in Escherichia coli and unexpected steady state kinetics of the phosphorylated forms. Biochemistry 44, 6286-92 15835917 Curated Info
5	Pekarsky Y, et al. (2004) Fhit is a physiological target of the protein kinase Src. Proc Natl Acad Sci U S A 101, 3775-9 15007172 Curated Info