Tyr657
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Home > Phosphorylation Site Page: > Tyr657  -  eNOS (human)

Site Information
FGLGSRAyPHFCAFA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2811400

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 4 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 4 ) , mutation of modification site ( 4 ) , phospho-antibody ( 1 , 2 , 3 , 4 ) , phosphoamino acid analysis ( 4 ) , phosphopeptide mapping ( 4 ) , western blotting ( 1 , 2 , 3 , 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 , 4 ) , endothelial ( 4 ) , endothelial-umbilical vein ( 3 ) , H9c2 (myoblast) ( 1 , 2 ) , heart ( 2 ) , HUVEC (endothelial) ( 4 ) , PAE (endothelial) ( 4 )

Upstream Regulation
Regulatory protein:
Src (human) ( 4 )
Putative in vivo kinases:
Pyk2 (human) ( 4 )
Treatments:
aminoxyacetic_acid ( 1 ) , fluid_shear_stress ( 4 ) , H2O2 ( 1 , 2 , 3 ) , insulin ( 4 ) , ischemia/reperfusion ( 2 ) , PF-431396 ( 2 ) , sodium sulfide ( 1 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, inhibited ( 2 , 3 , 4 )

References 

1

Bibli SI, et al. (2017) Hydrogen Sulfide Preserves Endothelial Nitric Oxide Synthase Function by Inhibiting Proline-Rich Kinase 2: Implications for Cardiomyocyte Survival and Cardioprotection. Mol Pharmacol 92, 718-730
29030392   Curated Info

2

Bibli SI, et al. (2017) Tyrosine phosphorylation of eNOS regulates myocardial survival after an ischaemic insult: role of PYK2. Cardiovasc Res 113, 926-937
28444132   Curated Info

3

Loot AE, Schreiber JG, Fisslthaler B, Fleming I (2009) Angiotensin II impairs endothelial function via tyrosine phosphorylation of the endothelial nitric oxide synthase. J Exp Med 206, 2889-96
19934023   Curated Info

4

Fisslthaler B, et al. (2008) Inhibition of endothelial nitric oxide synthase activity by proline-rich tyrosine kinase 2 in response to fluid shear stress and insulin. Circ Res 102, 1520-8
18483407   Curated Info