Thr200
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Home > Phosphorylation Site Page: > Thr200  -  NANOG (human)

Site Information
LPMWSNQtWNNSTWS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 28070707

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry (in vitro) ( 3 ) , mutation of modification site ( 3 ) , phospho-antibody ( 2 , 3 ) , western blotting ( 2 , 3 )
Disease tissue studied:
cancer, squamous cell carcinoma ( 3 )
Relevant cell line - cell type - tissue:
'stem, prostate cancer' ( 2 ) , 293 (epithelial) ( 3 ) , Cal27 (squamous) ( 3 ) , SCC-15 (squamous) ( 3 )

Upstream Regulation
Putative in vivo kinases:
PKCE (human) ( 3 )
Kinases, in vitro:
PKCE (human) ( 3 )
Treatments:
ibuprofen ( 2 ) , MK-591 ( 2 ) , siRNA ( 3 )

Downstream Regulation
Effects of modification on NANOG:
molecular association, regulation ( 3 ) , protein stabilization ( 3 )
Effects of modification on biological processes:
carcinogenesis, induced ( 3 ) , cell growth, induced ( 3 ) , cell motility, induced ( 3 ) , transcription, induced ( 3 )
Induce interaction with:
DNA ( 3 ) , NANOG (human) ( 3 ) , p300 (human) ( 3 )

References 

1

Sarveswaran S, Varma NRS, Morisetty S, Ghosh J (2019) Inhibition of 5-lipoxygenase downregulates stemness and kills prostate cancer stem cells by triggering apoptosis via activation of c-Jun N-terminal kinase. Oncotarget 10, 424-436
30728896   Curated Info

2

Sarveswaran S, Varma N, Morisetty S, Ghosh J (2016) Inhibition of 5-lipoxygenase downregulates stemness and kills prostate cancer stem cells by triggering apoptosis via activation of c-Jun N-terminal kinase. Oncotarget
27880719   Curated Info

3

Xie X, et al. (2014) Phosphorylation of Nanog is essential to regulate Bmi1 and promote tumorigenesis. Oncogene 33, 2040-52
23708658   Curated Info