Ser166
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Home > Phosphorylation Site Page: > Ser166  -  RIPK1 (mouse)

Site Information
VASFKTWsKLTKEKD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 27116401

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 5 , 6 , 7 , 9 ) , mutation of modification site ( 7 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , western blotting ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 )
Disease tissue studied:
colorectal cancer ( 1 , 3 ) , colorectal carcinoma ( 1 , 3 ) , lymphoma ( 3 ) , fibrosarcoma of soft tissue ( 3 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 3 ) , HT-29 (intestinal) ( 1 , 3 ) , L929 (fibroblast) ( 3 ) , macrophage-bone marrow ( 1 , 4 , 7 ) , MEF (fibroblast) ( 5 , 6 , 7 , 8 , 9 ) , U-937 (myeloid) ( 3 )

Upstream Regulation
Regulatory protein:
DAPK1 (human) ( 1 ) , DAPK1 (mouse) ( 1 ) , SPATA2 (mouse) ( 8 )
Putative in vivo kinases:
RIPK1 (mouse) ( 9 )
Treatments:
LPS ( 4 ) , Necrostatin-1 ( 4 ) , SM-164 ( 6 ) , TNF ( 1 , 6 , 7 , 9 ) , Z-VAD-FMK ( 1 , 4 , 6 , 9 )

Downstream Regulation
Effects of modification on biological processes:
apoptosis, induced ( 1 , 8 )

References 

1

Wu YH, et al. (2020) Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation. Cell Death Dis 11, 305
32366830   Curated Info

2

Ariana A, et al. (2020) Tristetraprolin regulates necroptosis during tonic Toll-like receptor 4 (TLR4) signaling in murine macrophages. J Biol Chem
32094226   Curated Info

3

Moerke C, et al. (2018) The anticonvulsive Phenhydan suppresses extrinsic cell death. Cell Death Differ
30442947   Curated Info

4

Sarhan J, et al. (2018) Constitutive interferon signaling maintains critical threshold of MLKL expression to license necroptosis. Cell Death Differ
29786074   Curated Info

5

Dziedzic SA, et al. (2018) ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC. Nat Cell Biol 20, 58-68
29203883   Curated Info

6

Wang H, et al. (2017) PELI1 functions as a dual modulator of necroptosis and apoptosis by regulating ubiquitination of RIPK1 and mRNA levels of c-FLIP. Proc Natl Acad Sci U S A 114, 11944-11949
29078411   Curated Info

7

Dondelinger Y, et al. (2017) MK2 phosphorylation of RIPK1 regulates TNF-mediated cell death. Nat Cell Biol 19, 1237-1247
28920952   Curated Info

8

Wei R, et al. (2017) SPATA2 regulates the activation of RIPK1 by modulating linear ubiquitination. Genes Dev 31, 1162-1176
28701375   Curated Info

9

Newton K, et al. (2016) RIPK1 inhibits ZBP1-driven necroptosis during development. Nature 540, 129-133
27819682   Curated Info