Ser103
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Home > Phosphorylation Site Page: > Ser103  -  SRF (human)

Site Information
RGLKRsLsEMEIGMV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447692
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 13 , 17 , 18 ) , electrophoretic mobility shift ( 13 ) , mass spectrometry ( 1 , 2 , 6 , 7 , 8 , 9 , 11 , 14 ) , mutation of modification site ( 5 , 15 , 17 , 18 ) , peptide sequencing ( 18 ) , phospho-antibody ( 3 , 10 , 12 , 13 , 15 , 16 , 17 ) , phosphoamino acid analysis ( 17 , 18 ) , phosphopeptide mapping ( 17 , 18 ) , western blotting ( 3 , 10 , 12 , 15 , 17 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 10 , 12 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 16 ) , breast ( 1 ) , COS (fibroblast) ( 17 ) , ES (stem) ( 5 ) , HeLa (cervical) ( 3 , 16 , 18 ) , HOS (bone cell) ( 13 ) , Jurkat (T lymphocyte) ( 2 , 6 , 7 , 8 , 9 , 11 , 15 ) , Swiss 3T3 (fibroblast) ( 17 )

Upstream Regulation
Putative in vivo kinases:
MAPKAPK2 (human) ( 16 )
Kinases, in vitro:
CAMK2A (human) ( 17 ) , MAPKAPK2 (human) ( 16 ) , MSK1 (human) ( 4 ) , p90RSK (mouse) ( 17 )
Treatments:
AlF4- ( 16 ) , anisomycin ( 3 , 13 , 16 ) , antibody ( 15 ) , arsenite ( 13 , 16 ) , exercise ( 10 , 12 ) , NaF ( 16 ) , PD98059 ( 16 ) , SB202190 ( 16 ) , serum ( 16 )

Downstream Regulation
Effects of modification on SRF:
molecular association, regulation ( 3 , 17 , 18 )
Effects of modification on biological processes:
transcription, altered ( 15 )
Induce interaction with:
DNA ( 3 , 17 , 18 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

3

Novellasdemunt L, et al. (2013) PFKFB3 activation in cancer cells by the p38/MK2 pathway in response to stress stimuli. Biochem J 452, 531-43
23548149   Curated Info

4

Shimada M, Nakadai T, Fukuda A, Hisatake K (2010) cAMP-response element-binding protein (CREB) controls MSK1-mediated phosphorylation of histone H3 at the c-fos promoter in vitro. J Biol Chem 285, 9390-401
20089855   Curated Info

5

Blaker AL, Taylor JM, Mack CP (2009) PKA-dependent phosphorylation of serum response factor inhibits smooth muscle-specific gene expression. Arterioscler Thromb Vasc Biol 29, 2153-60
19778940   Curated Info

6

Possemato A (2009) CST Curation Set: 7130; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

7

Possemato A (2009) CST Curation Set: 7128; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

8

Possemato A (2009) CST Curation Set: 7124; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

9

Zhou J (2009) CST Curation Set: 6541; Year: 2009; Biosample/Treatment: cell line, Jurkat/untreated; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

10

Rose AJ, et al. (2007) Effect of endurance exercise training on Ca2+ calmodulin-dependent protein kinase II expression and signalling in skeletal muscle of humans. J Physiol 583, 785-95
17627985   Curated Info

11

Possemato A (2007) CST Curation Set: 2648; Year: 2007; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

12

Rose AJ, Kiens B, Richter EA (2006) Ca2+-calmodulin-dependent protein kinase expression and signalling in skeletal muscle during exercise. J Physiol 574, 889-903
16690701   Curated Info

13

Bébien M, et al. (2003) Immediate-early gene induction by the stresses anisomycin and arsenite in human osteosarcoma cells involves MAPK cascade signaling to Elk-1, CREB and SRF. Oncogene 22, 1836-47
12660819   Curated Info

14

Chalkley RJ, Burlingame AL (2003) Identification of novel sites of O-N-acetylglucosamine modification of serum response factor using quadrupole time-of-flight mass spectrometry. Mol Cell Proteomics 2, 182-90
12684542   Curated Info

15

Charvet C, et al. (2002) Vav1 couples T cell receptor to serum response factor-dependent transcription via a MEK-dependent pathway. J Biol Chem 277, 15376-84
11859076   Curated Info

16

Heidenreich O, et al. (1999) MAPKAP kinase 2 phosphorylates serum response factor in vitro and in vivo. J Biol Chem 274, 14434-43
10318869   Curated Info

17

Rivera VM, et al. (1993) A growth factor-induced kinase phosphorylates the serum response factor at a site that regulates its DNA-binding activity. Mol Cell Biol 13, 6260-73
8413226   Curated Info

18

Janknecht R, et al. (1992) Identification of multiple SRF N-terminal phosphorylation sites affecting DNA binding properties. EMBO J 11, 1045-54
1547771   Curated Info

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