Ser97
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser97  -  DARPP-32 (mouse)

Site Information
NLsENQAsEEEDELG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 457675

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 4 , 5 , 6 , 7 , 8 ) , modification-specific antibody ( 9 ) , mutation of modification site ( 9 , 11 ) , phospho-antibody ( 3 , 9 , 10 , 11 , 12 ) , western blotting ( 3 , 9 , 10 )
Disease tissue studied:
melanoma skin cancer ( 8 )
Relevant cell line - cell type - tissue:
'brain, neostriatum' ( 12 ) , 'brain, nucleus accumbens' ( 3 , 9 ) , 'brain, striatum' ( 3 , 9 , 10 , 11 ) , 293 (epithelial) ( 9 ) , brain ( 5 , 6 , 7 ) , heart ( 6 ) , kidney ( 6 ) , liver ( 1 , 4 ) , lung ( 6 ) , pancreas ( 6 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 8 )

Upstream Regulation
Putative upstream phosphatases:
PPP2CA (mouse) ( 9 )
Treatments:
alpha-BTX ( 12 ) , CNQX ( 12 ) , cocaine ( 11 ) , colforsin ( 9 ) , DHbetaE ( 12 ) , haloperidol ( 3 ) , isoflurane ( 10 ) , ketamine ( 10 ) , mecamylamine ( 12 ) , MK801 ( 12 ) , nicotine ( 12 ) , okadaic_acid ( 9 ) , propofol ( 10 ) , quinpirole ( 3 ) , raclopride ( 12 ) , SCH_23390 ( 12 ) , SKF81297 ( 9 ) , TTX ( 12 )

Downstream Regulation
Effects of modification on DARPP-32:
intracellular localization ( 9 ) , phosphorylation ( 9 )

References 

1

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Engmann O, Giralt A, Girault JA (2016) Acute drug-induced spine changes in the nucleus accumbens are dependent on β-adducin. Neuropharmacology 110, 333-42
27480796   Curated Info

4

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

5

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

6

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

7

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

8

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

9

Stipanovich A, et al. (2008) A phosphatase cascade by which rewarding stimuli control nucleosomal response. Nature 453, 879-84
18496528   Curated Info

10

Snyder GL, Galdi S, Hendrick JP, Hemmings HC (2007) General anesthetics selectively modulate glutamatergic and dopaminergic signaling via site-specific phosphorylation in vivo. Neuropharmacology 53, 619-30
17826804   Curated Info

11

Zhang Y, et al. (2006) Cocaine self-administration in mice is inversely related to phosphorylation at Thr34 (protein kinase A site) and Ser130 (kinase CK1 site) of DARPP-32. J Neurosci 26, 2645-51
16525043   Curated Info

12

Hamada M, et al. (2005) Nicotine regulates DARPP-32 (dopamine- and cAMP-regulated phosphoprotein of 32 kDa) phosphorylation at multiple sites in neostriatal neurons. J Pharmacol Exp Ther 315, 872-8
16040813   Curated Info