Thr850
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Home > Phosphorylation Site Page: > Thr850  -  MYPT1 (chicken)

Site Information
PREKRRstGVSFWTQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452075

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 3 ) , phospho-antibody ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
ROCK1 (human) ( 3 )
Kinases, in vitro:
PKACA (human) ( 1 ) , ROCK1 (human) ( 2 , 3 )
Phosphatases, in vitro:
PPP1CB (human) ( 1 )
Treatments:
calyculin_A ( 3 ) , LPA ( 3 ) , serum ( 3 ) , Y27632 ( 3 )

References 

1

Grassie ME, et al. (2012) Cross-talk between Rho-associated Kinase and Cyclic Nucleotide-dependent Kinase Signaling Pathways in the Regulation of Smooth Muscle Myosin Light Chain Phosphatase. J Biol Chem 287, 36356-69
22948155   Curated Info

2

Yuen S, Ogut O, Brozovich FV (2011) MYPT1 protein isoforms are differentially phosphorylated by protein kinase G. J Biol Chem 286, 37274-9
21890627   Curated Info

3

Murányi A, et al. (2005) Phosphorylation of Thr695 and Thr850 on the myosin phosphatase target subunit: inhibitory effects and occurrence in A7r5 cells. FEBS Lett 579, 6611-5
16297917   Curated Info