Thr695
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Home > Phosphorylation Site Page: > Thr695  -  MYPT1 (chicken)

Site Information
ARQSRRstQGVTLTD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448587

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 7 ) , immunoprecipitation ( 7 ) , mutation of modification site ( 3 ) , peptide sequencing ( 7 ) , phospho-antibody ( 3 ) , phosphoamino acid analysis ( 7 ) , western blotting ( 7 )
Relevant cell line - cell type - tissue:
A7r5 ('muscle, smooth') ( 3 ) , bladder ( 7 )

Upstream Regulation
Putative in vivo kinases:
DAPK3 (human) ( 7 )
Kinases, in vitro:
DAPK3 (human) ( 4 , 6 , 7 ) , ILK (human) ( 4 , 5 ) , PKACA (human) ( 1 ) , ROCK1 (human) ( 2 , 3 , 4 ) , ROCK2 (human) ( 7 )
Phosphatases, in vitro:
PPP1CB (human) ( 1 )
Treatments:
calyculin_A ( 3 ) , carbachol ( 7 ) , LPA ( 3 ) , serum ( 3 ) , Y27632 ( 3 )

Downstream Regulation
Effects of modification on MYPT1:
enzymatic activity, inhibited ( 4 )

References 

1

Grassie ME, et al. (2012) Cross-talk between Rho-associated Kinase and Cyclic Nucleotide-dependent Kinase Signaling Pathways in the Regulation of Smooth Muscle Myosin Light Chain Phosphatase. J Biol Chem 287, 36356-69
22948155   Curated Info

2

Yuen S, Ogut O, Brozovich FV (2011) MYPT1 protein isoforms are differentially phosphorylated by protein kinase G. J Biol Chem 286, 37274-9
21890627   Curated Info

3

Murányi A, et al. (2005) Phosphorylation of Thr695 and Thr850 on the myosin phosphatase target subunit: inhibitory effects and occurrence in A7r5 cells. FEBS Lett 579, 6611-5
16297917   Curated Info

4

Murányi A, et al. (2002) Phosphorylation of the myosin phosphatase target subunit by integrin-linked kinase. Biochem J 366, 211-6
12030846   Curated Info

5

Kiss E, et al. (2002) Integrin-linked kinase phosphorylates the myosin phosphatase target subunit at the inhibitory site in platelet cytoskeleton. Biochem J 365, 79-87
11931630   Curated Info

6

Borman MA, et al. (2002) Smooth muscle myosin phosphatase-associated kinase induces Ca2+ sensitization via myosin phosphatase inhibition. J Biol Chem 277, 23441-6
11976330   Curated Info

7

MacDonald JA, et al. (2001) Identification of the endogenous smooth muscle myosin phosphatase-associated kinase. Proc Natl Acad Sci U S A 98, 2419-24
11226254   Curated Info