Thr395
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Home > Phosphorylation Site Page: > Thr395  -  CCNE1 (human)

Site Information
PLPSGLLtPPQsGKK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447676

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 12 , 16 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 3 , 6 , 7 , 9 , 11 ) , mutation of modification site ( 5 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ) , peptide sequencing ( 11 , 12 ) , phospho-antibody ( 1 , 11 , 12 ) , phosphoamino acid analysis ( 12 , 16 ) , phosphopeptide mapping ( 12 , 16 ) , western blotting ( 1 , 5 , 12 , 13 , 15 )
Disease tissue studied:
bone cancer ( 12 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , neuroblastoma ( 8 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CDK2 (human) ( 12 ) , GSK3A (human) ( 12 ) , GSK3B (human) ( 12 )
Kinases, in vitro:
CDK2 (human) ( 12 , 16 ) , GSK3B (human) ( 12 )
Treatments:
calyculin_A ( 1 ) , lithium ( 12 ) , LY294002 ( 12 ) , seliciclib ( 1 )

Downstream Regulation
Effects of modification on CCNE1:
molecular association, regulation ( 10 , 11 , 13 ) , protein degradation ( 5 , 10 , 11 , 13 , 14 , 16 ) , ubiquitination ( 15 )
Effects of modification on biological processes:
cell cycle regulation ( 13 , 16 )
Induce interaction with:
FBXW7 (human) ( 10 , 11 ) , SKP2 (human) ( 13 )

References 

1

Davis RJ, Swanger J, Hughes BT, Clurman BE (2017) The PP2A-B56 Phosphatase Opposes Cyclin E Autocatalytic Degradation via Site-Specific Dephosphorylation. Mol Cell Biol 37
28137908   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

4

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

5

Mull BB, Cox J, Bui T, Keyomarsi K (2009) Post-translational modification and stability of low molecular weight cyclin E. Oncogene 28, 3167-76
19561641   Curated Info

6

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

7

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
19276368   Curated Info

8

Otto T, et al. (2009) Stabilization of N-Myc is a critical function of Aurora A in human neuroblastoma. Cancer Cell 15, 67-78
19111882   Curated Info

9

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

10

Hao B, et al. (2007) Structure of a Fbw7-Skp1-cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligases. Mol Cell 26, 131-43
17434132   Curated Info

11

Ye X, et al. (2004) Recognition of phosphodegron motifs in human cyclin E by the SCF(Fbw7) ubiquitin ligase. J Biol Chem 279, 50110-9
15364936   Curated Info

12

Welcker M, et al. (2003) Multisite phosphorylation by Cdk2 and GSK3 controls cyclin E degradation. Mol Cell 12, 381-92
14536078   Curated Info

13

Yeh KH, et al. (2001) The F-box protein SKP2 binds to the phosphorylated threonine 380 in cyclin E and regulates ubiquitin-dependent degradation of cyclin E. Biochem Biophys Res Commun 281, 884-90
11237742   Curated Info

14

Yam CH, Siu WY, Lau A, Poon RY (2000) Degradation of cyclin A does not require its phosphorylation by CDC2 and cyclin-dependent kinase 2. J Biol Chem 275, 3158-67
10652300   Curated Info

15

Singer JD, Gurian-West M, Clurman B, Roberts JM (1999) Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. Genes Dev 13, 2375-87
10500095   Curated Info

16

Won KA, Reed SI (1996) Activation of cyclin E/CDK2 is coupled to site-specific autophosphorylation and ubiquitin-dependent degradation of cyclin E. EMBO J 15, 4182-93
8861947   Curated Info