Ser446
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Home > Phosphorylation Site Page: > Ser446  -  IRF5 (human)

Site Information
DsIRLQIsNPDLKDR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 26389901

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 ) , mutation of modification site ( 2 , 3 , 4 ) , phospho-antibody ( 2 ) , western blotting ( 2 , 3 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 ) , leukemia ( 2 ) , fibrosarcoma of soft tissue ( 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , breast ( 1 ) , E.coli (bacterial) ( 4 ) , Gen2.2 (dendritic cell) ( 2 ) , HT1080 (fibroblast) ( 3 )

Upstream Regulation
Putative in vivo kinases:
IKKB (human) ( 2 )
Kinases, in vitro:
IKKB (human) ( 2 )
Treatments:
CL097 ( 2 )

Downstream Regulation
Effects of modification on IRF5:
intracellular localization ( 2 , 3 ) , molecular association, regulation ( 4 ) , protein stabilization ( 4 )
Effects of modification on biological processes:
apoptosis, induced ( 3 ) , transcription, induced ( 3 )
Induce interaction with:
CBP (human) ( 4 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Lopez-Pelaez M, et al. (2014) Protein kinase IKK╬▓-catalyzed phosphorylation of IRF5 at Ser462 induces its dimerization and nuclear translocation in myeloid cells. Proc Natl Acad Sci U S A 111, 17432-7
25326418   Curated Info

3

Chang Foreman HC, Van Scoy S, Cheng TF, Reich NC (2012) Activation of interferon regulatory factor 5 by site specific phosphorylation. PLoS One 7, e33098
22412986   Curated Info

4

Chen W, et al. (2008) Insights into interferon regulatory factor activation from the crystal structure of dimeric IRF5. Nat Struct Mol Biol 15, 1213-20
18836453   Curated Info